The respiratory chain inhibitor rotenone affects peroxisomal dynamics via its microtubule-destabilising activity
| Autor: | Michael Schrader, Josiah B. Passmore, Maria Gomez-Lazaro, Sónia Pinho |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Histology Short Communication Respiratory chain Biology Mitochondrion medicine.disease_cause Microtubules Electron Transport 03 medical and health sciences chemistry.chemical_compound Microtubule Rotenone Chlorocebus aethiops Organelle Peroxisomes medicine Animals Inner mitochondrial membrane Molecular Biology Cells Cultured Uncoupling Agents ROS Cell Biology Peroxisome Mitochondria Cell biology Medical Laboratory Technology 030104 developmental biology chemistry COS Cells Mitochondrial Membranes Organelle cooperation Reactive Oxygen Species Oxidative stress |
| Zdroj: | Histochemistry and Cell Biology |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/s00418-017-1577-1 |
| Popis: | Peroxisomes and mitochondria in mammalian cells are closely linked subcellular organelles, which maintain a redox-sensitive relationship. Their interplay and role in ROS signalling are supposed to impact on age-related and degenerative disorders. Whereas the generation of peroxisome-derived oxidative stress can affect mitochondrial morphology and function, little is known about the impact of mitochondria-derived oxidative stress on peroxisomes. Here, we investigated the effect of the mitochondrial complex I inhibitor rotenone on peroxisomal and mitochondrial membrane dynamics. We show that rotenone treatment of COS-7 cells alters peroxisome morphology and distribution. However, this effect is related to its microtubule-destabilising activity rather than to the generation of oxidative stress. Rotenone also induced alterations in mitochondrial morphology, which—in contrast to its effect on peroxisomes—were dependent on the generation of ROS but independent of its microtubule-active properties. The importance of our findings for the peroxisome-mitochondria redox relationship and the interpretation of in cellulo and in vivo studies with rotenone, which is widely used to study Parkinson’s disease, are discussed. |
| Databáze: | OpenAIRE |
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