Lipid Accumulation in Peripheral Blood Dendritic Cells and Anticancer Immunity in Patients with Lung Cancer

Autor: Yoshiki Ishii, Tomoe Funakoshi, Ryo Arai, Tomoko Okutomi, Fumito Ohmi, Akihiro Takemasa, Sayo Soda, Hiroko Morita
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Myeloid
Lung Neoplasms
medicine.medical_treatment
Cell Separation
0302 clinical medicine
Immunology and Allergy
Cells
Cultured
Aged
80 and over
education.field_of_study
Antigen Presentation
medicine.diagnostic_test
Chemistry
Immunosuppression
hemic and immune systems
General Medicine
Middle Aged
Flow Cytometry
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
Research Article
Boron Compounds
lcsh:Immunologic diseases. Allergy
Article Subject
Immunology
Antigen presentation
Population
chemical and pharmacologic phenomena
Flow cytometry
03 medical and health sciences
medicine
Humans
education
Lung cancer
Triglycerides
Aged
Neoplasm Staging
Immunosuppression Therapy
Blood Cells
Cancer
Lipid metabolism
Dendritic Cells
medicine.disease
Lipid Metabolism
030104 developmental biology
Cancer research
Tumor Escape
Lymphocyte Culture Test
Mixed
lcsh:RC581-607
Zdroj: Journal of Immunology Research, Vol 2018 (2018)
Journal of Immunology Research
ISSN: 2314-7156
2314-8861
Popis: We studied the subsets of peripheral blood dendritic cells (DCs) and lipid accumulation in DCs to investigate the involvement of DCs in the decreased anticancer immunity of advanced lung cancer patients. We analyzed the population of DC subsets in peripheral blood using flow cytometry. We then determined lipid accumulation in the DCs using BODIPY 650/665, a fluorophore with an affinity for lipids. Compared with healthy controls, the number of DCs in the peripheral blood of treatment-naive cancer patients was significantly reduced. In patients with stage III + IV disease, the numbers of myeloid DCs (mDCs) and plasmacytoid DCs were also significantly reduced. Lipid accumulation in DCs evaluated based on the fluorescence intensity of BODIPY 650/665 was significantly higher in stage III + IV lung cancer patients than in the controls. In the subset analysis, the fluorescence was highest for mDCs. The intracellularly accumulated lipids were identified as triglycerides. A decreased mixed leukocyte reaction was observed in the mDCs from lung cancer patients compared with those from controls. Taken together, the results show that lung cancer patients have a notably decreased number of peripheral blood DCs and their function as antigen-presenting cells is decreased due to their high intracellular lipid accumulation. Thereby, anticancer immunity is suppressed.
Databáze: OpenAIRE
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