The assessment of xenogeneic bone immunotoxicity and risk management study
| Autor: | Xin Wang, Xiaoxia Sun, Chunling Li, Chenghu Liu, Likui Sun, Li Hou, Yanping Shi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cellular immunity lcsh:Medical technology Lymphocyte Transplantation Heterologous Biomedical Engineering Bone healing 030230 surgery Risk Assessment Biomaterials Mice 03 medical and health sciences 0302 clinical medicine Immune system Animals Immunotoxicity Medicine Radiology Nuclear Medicine and imaging Cell Proliferation Bone Transplantation Radiological and Ultrasound Technology business.industry Research Xenogeneic bone Regeneration (biology) Immunogenicity General Medicine Lymphocyte Subsets 030104 developmental biology medicine.anatomical_structure lcsh:R855-855.5 Risk management Cancellous Bone Humoral immunity Immunology Leukocytes Mononuclear Cattle Safety business Cancellous bone Immune safety |
| Zdroj: | BioMedical Engineering BioMedical Engineering OnLine, Vol 18, Iss 1, Pp 1-14 (2019) |
| ISSN: | 1475-925X |
| DOI: | 10.1186/s12938-019-0729-z |
| Popis: | Background Xenogeneic bone has been widely used in a variety of clinical bone-related disease to promote bone healing and restore bone defects. However, the adverse effects of immune system limit its application in the clinic. The aim of this study was to evaluate xenogeneic bone safety of immunotoxicity and explore the methods for immune risk supervision. Results Xenogeneic bone, which is freeze-dried bovine cancellous bone, was implanted into the muscle of mice. On day 7, 14 and 28, the effects of xenogeneic bone were examined on humoral immunity and cellular immunity, including the levels of IgG, IgM, C3, inflammatory factors (TNF-α, IL-6), alkaline phosphatase (ALP) and the lymphocyte phenotype. The data showed that xenogeneic bone implantation had no potential to induce immune responses not only in humoral immunity but also in cellular immunity. To reveal the risk of immunogenicity, the residual DNA and the clearance of α-gal epitope were analyzed in 2 different bones (bone 1 is deproteinized bone, bone 2 is acellular and defatted bone). It was suggested that DNA of xenogeneic bone can be limited to Conclusions Through the detection of xenogeneic bone immunotoxicity, our findings indicated that the supervisions of risk factors could contribute to reduce the immune risk. And the risk factors under the acceptable limitation could decrease or replace animal experiment. However, it still needs to be studied on the limitation of α-gal epitope to predict rejection of xenogeneic bone more accurately. |
| Databáze: | OpenAIRE |
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