Ectopically tethered CP190 induces large-scale chromatin decondensation
| Autor: | Rainer Renkawitz, Yogesh S. Shouche, Katharina Günther, Oliver Weth, Marek Bartkuhn, Ramesh R. Bhonde, Sajad H. Ahanger |
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| Rok vydání: | 2014 |
| Předmět: |
Transcriptional Activation
Nucleosome organization Insulator (genetics) Transfection Article Chromatin remodeling Cell Line Cell Line Tumor Animals Drosophila Proteins Humans Epigenetics ChIA-PET Mammals Regulation of gene expression Multidisciplinary biology Nuclear Proteins Chromatin Cell biology DNA-Binding Proteins HEK293 Cells Histone biology.protein Drosophila Insulator Elements Microtubule-Associated Proteins Protein Binding |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep03917 |
| Popis: | Insulator mediated alteration in higher-order chromatin and/or nucleosome organization is an important aspect of epigenetic gene regulation. Recent studies have suggested a key role for CP190 in such processes. In this study, we analysed the effects of ectopically tethered insulator factors on chromatin structure and found that CP190 induces large-scale decondensation when targeted to a condensed lacO array in mammalian and Drosophila cells. In contrast, dCTCF alone, is unable to cause such a decondensation, however, when CP190 is present, dCTCF recruits it to the lacO array and mediates chromatin unfolding. The CP190 induced opening of chromatin may not be correlated with transcriptional activation, as binding of CP190 does not enhance luciferase activity in reporter assays. We propose that CP190 may mediate histone modification and chromatin remodelling activity to induce an open chromatin state by its direct recruitment or targeting by a DNA binding factor such as dCTCF. |
| Databáze: | OpenAIRE |
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