Development of human Th1 and Th2 cytokine responses: The cytokine production profile of T cells is dictated by the primaryin vitro stimulus
| Autor: | Rolien de Jong, Lucien A. Aarden, Tineke van der Pouw-Kraan |
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| Rok vydání: | 1993 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte medicine.medical_specialty medicine.medical_treatment T cell Immunology CD2 Antigens Stimulation Biology Lymphocyte Activation chemistry.chemical_compound CD28 Antigens Antigens CD Internal medicine medicine Humans Immunology and Allergy Receptors Immunologic Cells Cultured Interleukin 4 CD28 Interleukin T-Lymphocytes Helper-Inducer T lymphocyte Molecular biology Cytokine medicine.anatomical_structure Endocrinology chemistry Phorbol Interleukin-2 Tetradecanoylphorbol Acetate Interleukin-4 |
| Zdroj: | European Journal of Immunology. 23:1-5 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830230102 |
| Popis: | Regulation of interleukin (IL)-4 production, but not IL-2 production, was found to be quite different in either freshly isolated T cells or T cell clones. Both fresh T cells and T helper 2-like clones produced IL-4 when stimulated with anti-CD2 in combination with anti-CD28. However, whereas T cell clones showed enhanced IL-4 production when phorbol 12-myristate 13-acetate (PMA) was used in addition to anti-CD2 and anti-CD28, IL-4 production by fresh T cells was inhibited by the presence of PMA. Prestimulation of fresh T cells led to the following observations: (a) activation in the absence of PMA led to a reversal of the PMA effect and (b) within 2 days these cells resembled T cell clones in that IL-4 production was no longer inhibited by PMA. When prestimulation was carried out in the presence of PMA, the inhibition of IL-4 production seemed irreversible. Removal of PMA after 3 days did not lead to renewed capability of IL-4 production, whereas IL-2 production was unimpaired. Our data show that the capacity of cultured T cells to produce IL-4 is determined and fixed during the first 2-3 days of stimulation. |
| Databáze: | OpenAIRE |
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