The Jasmonate-Responsive Element from the ORCA3 Promoter from Catharanthus roseus is Active in Arabidopsis and is Controlled by the Transcription Factor AtMYC2
| Autor: | Grégory Montiel, Johan Memelink, Ana P. Körbes, Adel Zarei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Transcriptional Activation
0106 biological sciences Catharanthus Physiology Molecular Sequence Data Arabidopsis Cyclopentanes Plant Science Response Elements 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Gene expression Botany Arabidopsis thaliana Oxylipins Jasmonate Transcription factor Plant Proteins 030304 developmental biology Regulation of gene expression 0303 health sciences Reporter gene Base Sequence biology Arabidopsis Proteins Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Chemistry fungi Cell Biology General Medicine Catharanthus roseus Plants Genetically Modified biology.organism_classification Cell biology Protein Biosynthesis Protein Binding Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant and Cell Physiology |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcr016 |
| Popis: | Jasmonates are plant signaling molecules that play key roles in protection against certain pathogens and against insects by switching on the expression of genes encoding defense proteins including enzymes involved in the biosynthesis of toxic secondary metabolites. In Catharanthus roseus, the ethylene response factor (ERF) transcription factor ORCA3 controls the jasmonate-responsive activation of terpenoid indole alkaloid biosynthetic genes. ORCA3 gene expression is itself induced by jasmonate. Its promoter contains an autonomous jasmonate-responsive element (JRE). Here we describe the jasmonate-responsive activity of the JRE from the ORCA3 promoter in Arabidopsis thaliana. We found that it interacts in vitro and in vivo with the basic helix-loop-helix transcription factor AtMYC2. Analysis of JRE-mediated reporter gene expression in an atmyc2-1 mutant background showed that the activity was strictly dependent on AtMYC2. |
| Databáze: | OpenAIRE |
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