Studies on the Underhydroxylated Basement Membrane Procollagen Synthesized by Rat Parietal Yolk Sacs in the Presence of α, α'-Dipydridyl
| Autor: | Nicholas A. Kefalides, Marlene W. Karakashian |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
food.ingredient
Proline Pyridines Sodium Size-exclusion chromatography chemistry.chemical_element macromolecular substances Biology Hydroxylation Biochemistry Basement Membrane 2 2'-Dipyridyl food Rheumatology Culture Techniques Yolk medicine Animals Chymotrypsin Orthopedics and Sports Medicine Disulfides Molecular Biology Yolk Sac Basement membrane integumentary system Rats Inbred Strains Embryo Cell Biology Rats Procollagen peptidase medicine.anatomical_structure chemistry Chromatography Gel Procollagen Type I collagen |
| Zdroj: | Connective Tissue Research. 10:247-262 |
| ISSN: | 1607-8438 0300-8207 |
| Popis: | Rat embryo parietal yolk sacs synthesized and secreted underhydroxylated [14C]proline-labeled basement membrane procollagen when incubated in the presence of the iron chelator alpha,alpha'-dipyridyl and [14C]proline. Upon reduction and sodium dodecyl sulfate-agarose gel filtration, this procollagen eluted as a discrete peak of labeled material close to the position of the beta components of type I collagen. Secreted underhydroxylated procollagen contained interchain disulfide bonds and was completely degraded by a 6 hr treatment with alpha-chymotrypsin at 4 degrees C. Results from 4 hr incubations indicated that the distribution of 14C-procollagen between tissue and medium was unaffected by the presence of alpha,alpha'-dipyridyl, but analysis of results from pulse-label and chase experiments revealed that underhydroxylated procollagen was secreted at less than half the rate of control procollagen. |
| Databáze: | OpenAIRE |
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