Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes
| Autor: | Anna Salvati, Beatrice Rossotti, Daniela Maggioni, Elisabetta Ranucci, Luca Mascheroni, Valentina Francia, Paolo Ferruti |
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| Přispěvatelé: | Nanomedicine & Drug Targeting, Nanotechnology and Biophysics in Medicine (NANOBIOMED), Biopharmaceuticals, Discovery, Design and Delivery (BDDD) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Light Endosome Cell Survival polyamidoamines 02 engineering and technology endosomal escape 010402 general chemistry Endocytosis 01 natural sciences light-triggered escape Ruthenium chemistry.chemical_compound Live cell imaging Coordination Complexes Cations luminescent ruthenium complexes medicine Polyamines Tumor Cells Cultured Humans General Materials Science nuclear trafficking Cell Nucleus Photosensitizing Agents Molecular Structure Singlet Oxygen Singlet oxygen Optical Imaging 021001 nanoscience & nanotechnology 0104 chemical sciences Cytosol medicine.anatomical_structure chemistry Biophysics Nanocarriers 0210 nano-technology Nucleus Intracellular HeLa Cells Research Article |
| Zdroj: | ACS Applied Materials & Interfaces ACS Applied Materials & Interfaces, 12(31), 34576-34587. AMER CHEMICAL SOC |
| ISSN: | 1944-8244 |
| Popis: | Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine (PAA) endowed with a luminescent Ru complex, Ru-PhenAN, that shows unique trafficking to the cell nucleus. Live cell imaging confirmed the capacity of this polymer to access the nucleus, excluding artifacts due to cell fixation, and clarified that the mechanism of escape is light-triggered and relies on the presence of the Ru complexes and their capacity to absorb light and act as photosensitizers for singlet oxygen production. These results open up the possibility to use PAA-ruthenium complexes for targeted light-triggered delivery of genetic material or drugs to the cytosol and nucleus. |
| Databáze: | OpenAIRE |
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