Direct biosensor immunoassays for the detection of nonmilk proteins in milk powder
| Autor: | Kees Olieman, Geert Cazemier, Willem Haasnoot, Ron Verheijen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
pea
Food Contamination Biosensing Techniques Biology soy medicine Animals Immunoassay Detection limit Chromatography medicine.diagnostic_test Milk protein Wageningen Food Safety Research Screening assay food and beverages General Chemistry Optical biosensor Milk Biochemistry Plant protein Polyclonal antibodies soluble wheat proteins biology.protein General Agricultural and Biological Sciences Biosensor plant proteins |
| Zdroj: | Journal of Agricultural and Food Chemistry, 49(11), 5201-5206 Journal of Agricultural and Food Chemistry 49 (2001) 11 |
| ISSN: | 0021-8561 |
| Popis: | The low prices of some nonmilk proteins make them attractive as potential adulterants in dairy products. An optical biosensor (BIACORE 3000) was used to develop a direct and combined biosensor immunoassay (BIA) for the simultaneous detection of soy, pea, and soluble wheat proteins in milk powders. Affinity-purified polyclonal antibodies raised against the three protein sources were immobilized in different flow channels (Fcs) on the biosensor chip (CM5). Dissolved milk powders were injected (20 microL injections at 20 microL min(-1)) through the serially connected Fcs, and the antibody-bound plant proteins were detected directly. The total run time between samples, including a regeneration step with 5 microL of 10 mM HCl, was 5 min. The limits of detection in milk powder were below 0.1% of plant protein in the total milk protein content. The antibodies also recognized some proteins from other plant sources, which made this BIA even more suitable as a broad screening assay for nonmilk proteins. |
| Databáze: | OpenAIRE |
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