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The aim of this study was to investigate the antifertility efficacy of betel leaf stalk extract on lipid metabolites. The betel leaf stalk extract was administered at the dose of 50 mg/Kg/day through oral gavages method for 15 days. The impaired lipid metabolisms were observed in testis. The higher lipids in seminal vesicle and prostate gland indicate the elevation in lipid metabolism and lipogenesis, suggesting some alterations in the chemical composition of the seminal plasma and prostatic fluid. The accumulation of lipids in prostate causes the inhibition in lipase activity. The elevated testicular Phospholipids due to administration of extract results the disruption in integrity of the spermatozoal membrane and its adequate phospholipid composition leads to infertility. Significant reductions in triglycerides represent no risk of cancer. Increased concentration of cholesterol in testes suggests the impairment of spermatogenesis. 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