In situ enrichment of folate by microorganisms in beta-glucan rich oat and barley matrices
Autor: | Hannu Salovaara, Laura Nyström, Matti Korhola, Minnamari Edelmann, Tuula Sontag-Strohm, Susanna Kariluoto, Reetta Kivelä, Mirkka Herranen, Vieno Piironen |
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Rok vydání: | 2013 |
Předmět: |
0106 biological sciences
Streptococcus thermophilus beta-Glucans Avena Propionibacterium 01 natural sciences Microbiology Beta-glucan chemistry.chemical_compound 0404 agricultural biotechnology Folic Acid 010608 biotechnology Yeasts 2. Zero hunger biology Viscosity food and beverages Hordeum 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science Yeast Lactobacillus reuteri Lactic acid chemistry Biochemistry Fermentation Food Microbiology Bacteria Food Science |
Zdroj: | International journal of food microbiology. 176 |
ISSN: | 1879-3460 |
Popis: | The objective was to study folate production of yeast strains, bacteria isolated from oat bran, and selected lactic acid bacteria as well as one propionibacterium in oat and barley based models. Simultaneously, we aimed at sustaining the stability of viscosity, representing the physicochemical state of beta-glucan. Total folate contents were determined microbiologically and vitamers for selected samples by UHPLC. Folate in yeast cells comprised mainly 5-methyltetrahydrofolate and tetrahydrofolate. Folate production by microbes in YPD medium was different to that in cereal fermentations where vitamers included 5-methyltetrahydrofolate, 5,10-methenyltetrahydrofolate and formylated derivatives. Microbes producing significant amounts of folate without affecting viscosity were Saccharomyces cerevisiae ALKO743 and Candida milleri ABM4949 among yeasts and Pseudomonas sp. ON8 and Janthinobacterium sp. RB4 among bacteria. Net folate production was up to 120 ng/g after 24 h fermentation and could increase during 2-week storage. Glucose addition increased the proportion of 5-methyltetrahydrofolate. Streptococcus thermophilus ABM5097, Lactobacillus reuteri, and Propionibacterium sp. ABM5378 produced folate but in lower concentrations. Both endogenous and added microbes contribute to folate enhancement. Selection of microbes with folate producing capability and limited hydrolytic activity will enable the development of products rich in folate and beta-glucan. |
Databáze: | OpenAIRE |
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