Vitamin E is essential for the tolerance of Arabidopsis thaliana to metal-induced oxidative stress

Autor: Françoise Eymery, Pascal Rey, Michel Havaux, Bernard Genty, Valérie Collin
Přispěvatelé: Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Jazyk: angličtina
Rok vydání: 2007
Předmět:
0106 biological sciences
Antioxidant
Physiology
medicine.medical_treatment
Blotting
Western
Arabidopsis
Tocopherols
Plant Science
Ascorbic Acid
medicine.disease_cause
01 natural sciences
Antioxidants
Lipid peroxidation
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
Malondialdehyde
medicine
Arabidopsis thaliana
Vitamin E
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
RNA
Messenger
Intramolecular Transferases
Chromatography
High Pressure Liquid
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
biology
Arabidopsis Proteins
food and beverages
Glutathione
Ascorbic acid
biology.organism_classification
beta Carotene
Adaptation
Physiological
Phosphoric Monoester Hydrolases
Oxidative Stress
chemistry
Biochemistry
Luminescent Measurements
Mutation
Lipid Peroxidation
Oxidative stress
Copper
010606 plant biology & botany
Cadmium
Zdroj: Plant, Cell and Environment
Plant, Cell and Environment, Wiley, 2007, pp.071202154113001-???. ⟨10.1111/j.1365-3040.2007.01755.x⟩
Plant, Cell and Environment, 2007, pp.071202154113001-???. ⟨10.1111/j.1365-3040.2007.01755.x⟩
ISSN: 0140-7791
1365-3040
DOI: 10.1111/j.1365-3040.2007.01755.x⟩
Popis: Arabidopsis (Arabidopsis thaliana) plants were grown in a hydroponic culture system for 7 to 14 d in the absence or presence of 75 microM Cd or 75 microM Cu. The Cu treatment resulted in visual leaf symptoms, together with anthocyanin accumulation and loss of turgor. Pronounced lipid peroxidation, which was detected by autoluminescence imaging and malondialdehyde titration, was observed in Cu-treated leaves. The Cd treatment also resulted in loss of leaf pigments but lipid peroxidation and oxidative stress were less pronounced than in the leaves exposed to Cu. Analysis of low-molecular-weight chloroplast and cytosolic antioxidants (ascorbate, glutathione, tocopherols, carotenoids) and antioxidant enzymes (thiol-based reductases and peroxidases) revealed relatively few responses to metal exposure. However, there was a marked increase in vitamin E (alpha-tocopherol) in response to Cd and Cu treatments. Ascorbate increased significantly in Cu-exposed leaves. Other antioxidants either remained stable or decreased in response to metal stress. Transcripts encoding enzymes of the vitamin E biosynthetic pathway were increased in response to metal exposure. In particular, VTE2 mRNA was enhanced in Cu- and Cd-treated plants, while VTE5 and hydroxylpyruvate dioxygenase (HPPD) mRNAs were only up-regulated in Cd-treated plants. Consistent increases in HPPD transcripts and protein were observed. The vitamin E-deficient (vte1) mutant exhibited an enhanced sensitivity towards both metals relative to the wild-type (WT) control. Unlike the vte1 mutants, which showed enhanced lipid peroxidation and oxidative stress in the presence of Cu or Cd, the ascorbate-deficient (vtc2) mutant showed WT responses to metal exposure. Taken together, these results demonstrate that vitamin E plays a crucial role in the tolerance of Arabidopsis to oxidative stress induced by heavy metals such as Cu and Cd.
Databáze: OpenAIRE
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