Temperature and nutrient limitations decrease transfer of conjugative IncP-1 plasmid pKJK5 to wild Escherichia coli strains
| Autor: | Pallares-Vega, Rebeca, Macedo, Gonçalo, Brouwer, Michael S M, Hernandez Leal, Lucia, van der Maas, Peter, van Loosdrecht, Mark C M, Weissbrodt, David G, Heederik, Dick, Mevius, Dik, Schmitt, Heike, Sub Research support office, Faculteit Diergeneeskunde, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I RA, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I I&I |
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| Přispěvatelé: | Sub Research support office, Faculteit Diergeneeskunde, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I RA, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I I&I |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Epidemiology Bioinformatica & Diermodellen Microorganism medicine.disease_cause Microbiology 03 medical and health sciences Antibiotic resistance Plasmid Bodem Bio-informatics & Animal models Afvalwater medicine soil extract agar Epidemiology Bio-informatics & Animal models AMR antimicrobial resistance Food science Psychrophile Escherichia coli Original Research 030304 developmental biology Host Pathogen Interaction & Diagnostics Epidemiologie 0303 health sciences Strain (chemistry) biology 030306 microbiology Chemistry Bacteriologie Bacteriology Bacteriology Host Pathogen Interaction & Diagnostics biology.organism_classification Enterobacteriaceae Host Pathogen Interactie & Diagnostiek QR1-502 environmental conditions Epidemiologie Bioinformatica & Diermodellen Bacteriologie Host Pathogen Interactie & Diagnostiek Horizontal gene transfer horizontal gene transfer Resistentie synthetic wastewater |
| Zdroj: | Frontiers in Microbiology, 12(656250). [S.l.]: Frontiers Media Frontiers in Microbiology 12 (2021) Frontiers in Microbiology, 12, 1. Frontiers Media S.A. Frontiers in Microbiology, 12 Frontiers in Microbiology Frontiers in Microbiology, Vol 12 (2021) |
| ISSN: | 1664-302X |
| Popis: | Plasmid-mediated dissemination of antibiotic resistance among fecal Enterobacteriaceae in natural ecosystems may contribute to the persistence of antibiotic resistance genes in anthropogenically impacted environments. Plasmid transfer frequencies measured under laboratory conditions might lead to overestimation of plasmid transfer potential in natural ecosystems. This study assessed differences in the conjugative transfer of an IncP-1 (pKJK5) plasmid to three natural Escherichia coli strains carrying extended-spectrum beta-lactamases, by filter mating. Matings were performed under optimal laboratory conditions (rich LB medium and 37°C) and environmentally relevant temperatures (25, 15 and 9°C) or nutrient regimes mimicking environmental conditions and limitations (synthetic wastewater and soil extract). Under optimal nutrient conditions and temperature, two recipients yielded high transfer frequencies (5 × 10–1) while the conjugation frequency of the third strain was 1000-fold lower. Decreasing mating temperatures to psychrophilic ranges led to lower transfer frequencies, albeit all three strains conjugated under all the tested temperatures. Low nutritive media caused significant decreases in transconjugants (−3 logs for synthetic wastewater; −6 logs for soil extract), where only one of the strains was able to produce detectable transconjugants. Collectively, this study highlights that despite less-than-optimal conditions, fecal organisms may transfer plasmids in the environment, but the transfer of pKJK5 between microorganisms is limited mainly by low nutrient conditions. |
| Databáze: | OpenAIRE |
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