Visualisation of phenotypically mixed HIV-1 and HTLV-I virus particles by electron microscopy
| Autor: | Dale A. McPhee, Victoria A. Lawson, J.Y. Lee, John A Marshall, Jennifer C. Doultree |
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| Rok vydání: | 2000 |
| Předmět: |
viruses
Endocrinology Diabetes and Metabolism Clinical Biochemistry Retroviridae Proteins Biology Antibodies Viral Virus law.invention law Labelling Humans Pharmacology (medical) Molecular Biology Tropism Cell Line Transformed chemistry.chemical_classification Human T-lymphotropic virus 1 Biochemistry (medical) Virion virus diseases Cell Biology General Medicine Immunogold labelling biology.organism_classification Immunohistochemistry Precipitin Tests Virology Molecular biology In vitro Microscopy Electron Phenotype chemistry HIV-1 Electron microscope Glycoprotein |
| Zdroj: | Journal of Biomedical Science. 7:71-74 |
| ISSN: | 1423-0127 1021-7770 |
| DOI: | 10.1007/bf02255921 |
| Popis: | Virions produced after HIV-1 infection of HTLV-I transformed cells have an expanded tropism that has been attributed to the presence of HTLV-I glycoproteins in the envelope. This report now directly identifies these phenotypically mixed virions by immunogold labelling electron microscopy. Furthermore we estimate there are 2% of these in cell-free supernatant, which represents up to 1 x 10(7) particles/ml from an in vitro infection. HTLV-1 envelope labelling was localised to a single region, suggesting a defined event in packaging of foreign envelope proteins into HIV-1 virus particles. |
| Databáze: | OpenAIRE |
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