The immune microenvironment in cutaneous leishmaniasis
| Autor: | Ibrahim Khalifeh, Haifaa Khalifeh, Mark Jabbour, Robert H. Habib, Khalil M. Charafeddine, Y. Simaan, Grace Issa, M. Karam |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology medicine.medical_specialty Leishmania tropica Adolescent CD3 Complex T-Lymphocytes Antigens Differentiation Myelomonocytic Leishmaniasis Cutaneous Dermatology Antigens CD1 Young Adult Immune system Cutaneous leishmaniasis Dermis Antigen Antigens CD Humans Medicine Child Leishmania major integumentary system biology business.industry Macrophages Leishmaniasis biology.organism_classification medicine.disease Leishmania Infectious Diseases medicine.anatomical_structure Cellular Microenvironment Langerhans Cells Immunology Female Epidermis business CD8 |
| Zdroj: | Journal of the European Academy of Dermatology and Venereology. 29:1170-1179 |
| ISSN: | 0926-9959 |
| DOI: | 10.1111/jdv.12781 |
| Popis: | Background Cutaneous leishmaniasis is an infection that has spread to non-endemic regions, stimulating recent interest for the enhanced understanding of this disease. Downregulation of the CD1a receptor on Langerhans cells has been described in various cutaneous infections. Objective In this study, the immune response across different Ridley patterns and parasitic indices is outlined in a case series of cutaneous leishmaniasis. Methods Skin punch biopsies from the interface of normal and lesional cutaneous leishmaniasis were collected from 33 patients with molecularly confirmed Leishmania tropica or L. major infection. Ridley patterns (2–5) were assessed for various clinicopathological features including age, gender, disease duration, parasitic index and constituents of the inflammatory infiltrate. CD1a, CD68, CD3, CD4, CD8, CD20 and CD138 stains were performed on normal skin tissue, cutaneous leishmaniasis biopsies and cytospin/cell block cytology preparations of cultured leishmania promastigotes. CD1a was quantified per mm² in the epidermis and dermis. The remaining stains were graded according to a 4-tiered grading system [0 (0–4%); 1 (5–24%); 2 (25–49%); 3 (50–74%) and 4 (75–100%). Results Total CD1a expression significantly decreased (14-fold) from parasitic indices (0–2) to (5–6); (ρ |
| Databáze: | OpenAIRE |
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