Establishing an allergic eczema model employing recombinant house dust mite allergens D er p 1 and D er p 2 in BALB /c mice
| Autor: | Philipp Starkl, Luc Vander Elst, Caroline Stremnitzer, Jean-Marie Saint-Remy, Tamara Kopp, Julia Wallmann, Isabella Pali-Schöll, Krisztina Szalai, Erika Jensen-Jarolim, Anna Lukschal |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
T-Lymphocytes
atopic eczema recombinant allergens Dermatology Immunoglobulin E medicine.disease_cause Biochemistry Der p1 & Der p 2 Arthropod Proteins Dermatitis Atopic BALB/c Mice 03 medical and health sciences 0302 clinical medicine Allergen Antigen immune system diseases medicine Animals allergic eczema Antigens Dermatophagoides Mast Cells house dust mite Molecular Biology 030304 developmental biology House dust mite Mice Inbred BALB C 0303 health sciences biology Pyroglyphidae Original Articles Allergens Eosinophil biology.organism_classification Recombinant Proteins 3. Good health Eosinophils Cysteine Endopeptidases Disease Models Animal medicine.anatomical_structure 030228 respiratory system Immunoglobulin G Immunology biology.protein protease activity Female Antibody |
| Zdroj: | Experimental Dermatology |
| ISSN: | 1600-0625 0906-6705 |
| DOI: | 10.1111/exd.12015 |
| Popis: | The major house dust mite allergens Der p 1 and Der p 2 are prevalent inducers of eczema. Der p 1 is a cysteine protease disrupting epithelial barriers, whereas Der p 2 functionally mimics the LPS-binding compound MD-2 within the TLR4 complex. In this work, we tested the percutaneous sensitizing capacity of recombinant (r) Der p 1 and Der p 2 in BALB/c mice. Mice were sensitized by percutaneous application of low (10 μg/application) and high dose (100 μg) rDer p 1 or rDer p 2, or with rDer p 1 followed by rDer p 2. Allergen-specific and total IgE antibodies were determined by ELISA. Eczema of BALB/c was classified by the itching score and corresponded to erosions. Infiltrating immune cells were identified by haematoxylin/eosin and Giemsa staining for eosinophils or mast cells, CD3 staining for T lymphocytes. Percutaneous treatments with rDer p 1, but not rDer p 2-induced specific IgG1. However, cotreatment with rDer p 1 led to increase in anti-Der p 2 IgG titres. Both allergens elicited skin erosions because of scratching, thickening of the epidermis, and eosinophil and T-cell infiltration. Our data indicate that recombinant mite allergens in the absence of adjuvant are sufficient for inducing eczema in BALB/c mice. As the enzymatic activity of an allergen might be an important cofactor for specific sensitization via the skin, Der p 1 may act as adjuvant for other allergens too. The presented mouse model is suitable for investigating the mechanisms of allergic eczema. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|