Maternal total cell-free DNA in preeclampsia and fetal growth restriction: Evidence of differences in maternal response to abnormal implantation

Autor:	Vered Klaitman, Salvatore Andrea Mastrolia, Dan Tirosh, Shirley Greenbaum, Offer Erez, Alex Alioshin, Neta Benshalom-Tirosh, Amos Douvdevani, Reut Riff, Giuseppe Loverro, Majdi Imterat, Tal Rafaeli-Yehudai, Ruthy Beer-Weisel, Mariana Rita Catalano, Gal Rodavsky Hanegbi
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	Percentile Embryology Neutrophils Maternal Health Placenta lcsh:Medicine Apoptosis Pathology and Laboratory Medicine Pediatrics White Blood Cells 0302 clinical medicine Child Development Pre-Eclampsia Pregnancy Animal Cells Medicine and Health Sciences Prospective Studies lcsh:Science Prospective cohort study Immune Response 030219 obstetrics & reproductive medicine Multidisciplinary Fetal Growth Retardation Cell Death Obstetrics and Gynecology Systemic Inflammatory Response Syndrome medicine.anatomical_structure Cell Processes 030220 oncology & carcinogenesis Female medicine.symptom Anatomy Cellular Types Cell-Free Nucleic Acids Research Article Adult Child Growth Immune Cells Immunology Inflammation Ultrasonography Prenatal Preeclampsia Andrology 03 medical and health sciences Signs and Symptoms Diagnostic Medicine Sepsis medicine Humans Embryo Implantation Fetus Blood Cells Growth Restriction business.industry lcsh:R Reproductive System Biology and Life Sciences Ultrasonography Doppler Cell Biology medicine.disease Systemic inflammatory response syndrome Pregnancy Complications Cross-Sectional Studies Women's Health lcsh:Q business Biomarkers Developmental Biology
Zdroj:	PLoS ONE PLoS ONE, Vol 13, Iss 7, p e0200360 (2018)
ISSN:	1932-6203
Popis:	Objectives Preeclampsia and fetal growth restriction are obstetrical syndromes associated with abnormal placental implantation and changes in the activation status of maternal leukocytes. This study is aimed to determine by a simple, rapid fluorescent assay the changes in maternal serum total cell-free DNA (t-cfDNA) concentrations in women with preeclampsia and those with fetal growth restriction (FGR). Study design A cross-sectional study was conducted measuring maternal serum t-cfDNA concentrations. Women were classified into the following groups: 1) patients with preeclampsia (n = 21); 2) FGR-estimated fetal weight below the 10thpercentile (n = 28); and 3) normal pregnancy (n = 39). Serum samples were directly assayed for t-cfDNA using a rapid fluorescent SYBR Gold assay. Elevated maternal serum t-cfDNA concentrations were defined as a cutoff>850ng/ml. Nonparametric statistics were used for analysis. Results Women with preeclampsia had a higher median maternal serum concentration (802 ng/ml, 400–2272 ng/ml) than women with a normal pregnancy (499 ng/ml, 0–1892 ng/ml, p = 0.004) and those with FGR (484 ng/ml, 72–2187 ng/ml, p = 0.012). Moreover, even patients with FGR
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbce1e4687b848c4db9e3472307f8e00 http://europepmc.org/articles/PMC6042756 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.