Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications

Autor: Alexander R. Harris, John Will, Pia C. Winberg, Zhilian Yue, Gordon G. Wallace, Jeremy Dinoro, Paul J. Molino, Luciana Y. Daikuara
Rok vydání: 2021
Předmět:
Cell Survival
Polymers
General Physics and Astronomy
Biocompatible Materials
02 engineering and technology
010402 general chemistry
Microscopy
Atomic Force
01 natural sciences
Rhamnose
General Biochemistry
Genetics and Molecular Biology
Biomaterials
Contact angle
chemistry.chemical_compound
Adsorption
PEDOT:PSS
Polysaccharides
Electrochemistry
Humans
General Materials Science
Cell Shape
Conductive polymer
chemistry.chemical_classification
Wound Healing
Xylose
Chemistry
Electric Conductivity
Transferrin
Fibrinogen
General Chemistry
Quartz crystal microbalance
Dermis
Fibroblasts
021001 nanoscience & nanotechnology
Bridged Bicyclo Compounds
Heterocyclic
0104 chemical sciences
Uronic Acids
Chemical engineering
Dielectric Spectroscopy
Quartz Crystal Microbalance Techniques
Collagen
0210 nano-technology
Poly(3
4-ethylenedioxythiophene)
Protein adsorption
Zdroj: Biointerphases. 16(2)
ISSN: 1559-4106
Popis: We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes. Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle. PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode. A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced. The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings. Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.
Databáze: OpenAIRE
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