Transmission of Pneumocystis carinii DNA from a Patient with P. carinii Pneumonia to Immunocompetent Contact Health Care Workers
| Autor: | Maria P. Muñoz, Susana Prieto, Walter T. Hughes, Sergio L. Vargas, Carolina A. Ponce, Francis Gigliotti, Ana V. Ulloa |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Microbiology (medical) Infectious Disease Transmission Patient-to-Professional Health Personnel Mycology Polymerase Chain Reaction law.invention law Nasopharynx parasitic diseases medicine Pneumocystosis Humans DNA Fungal Antibodies Fungal Polymerase chain reaction Mycosis Pneumocystis business.industry Transmission (medicine) Pneumonia Pneumocystis Respiratory disease Infant bacterial infections and mycoses medicine.disease respiratory tract diseases Pneumocystis Infections Pneumonia Pneumocystis carinii Immunology Female business Immunocompetence |
| Zdroj: | Journal of Clinical Microbiology. 38:1536-1538 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.38.4.1536-1538.2000 |
| Popis: | The transmission of Pneumocystis carinii from person to person was studied by detecting P. carinii -specific DNA in prospectively obtained noninvasive deep-nasal-swab samples from a child with a documented P. carinii pneumonia (PCP), his mother, two contact health care workers, and 30 hospital staff members who did not enter the patient's room (controls). Nested-DNA amplification was done by using oligonucleotide primers designed for the gene encoding the mitochondrial large subunit rRNA of rat P. carinii ( P. carinii f. sp. carinii ) that amplifies all forms of P. carinii and internal primers specific for human P. carinii (f. sp. hominis ). P. carinii f. sp. hominis DNA was detected in samples from the patient and all of his contacts versus none of the 30 hospital staff members. The results, as previously shown in murine models of P. carinii pneumonia, document that person-to-person transmission of P. carinii is possible. This observation suggests that immunocompromised patients not on PCP prophylaxis should not enter the room of a patient with PCP, and it also raises the question as to whether healthy contacts can transmit the disease to immunocompromised patients at risk. |
| Databáze: | OpenAIRE |
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