Role of IFN-gamma and LPS on neuron/glial co-cultures infected by Neospora caninum
| Autor: | Erica Etelvina Viana de Jesus, Maria de Fátima Dias Costa, Alex Barbosa dos Santos, Cátia Suse de Oliveira Ribeiro, Songeli Menezes Freire, Silvia Lima Costa, Alexandre Moraes Pinheiro, Ramon dos Santos El-Bachá |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Neurite
medicine.medical_treatment neurite impairment Neospora caninum Neuroprotection immune response lcsh:RC321-571 Cellular and Molecular Neuroscience Immune system medicine Original Research Article Neuron/glia co-culture parasite NO down-modulation parasite NO downmodulation lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry biology Microglia neuron/glial co-culture biology.organism_classification medicine.anatomical_structure Cytokine Immunology Neuron Neuroscience Astrocyte |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA Frontiers in Cellular Neuroscience, Vol 8 (2014) Frontiers in Cellular Neuroscience |
| DOI: | 10.3389/fncel.2014.00340 |
| Popis: | Submitted by Ramon El-Bachá (ramon@ufba.br) on 2016-09-30T20:33:12Z No. of bitstreams: 1 de Jesus et al 2014.pdf: 915416 bytes, checksum: 4d7c829f8d4091e11a0ce03d46824d54 (MD5) Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2016-10-07T11:54:37Z (GMT) No. of bitstreams: 1 de Jesus et al 2014.pdf: 915416 bytes, checksum: 4d7c829f8d4091e11a0ce03d46824d54 (MD5) Made available in DSpace on 2016-10-07T11:54:37Z (GMT). No. of bitstreams: 1 de Jesus et al 2014.pdf: 915416 bytes, checksum: 4d7c829f8d4091e11a0ce03d46824d54 (MD5) Previous issue date: 2014-10-27 CNPq/CAPES Neospora caninum causes cattle abortion and neurological symptoms in dogs. Although infection is usually asymptomatic, classical neurological symptoms of neosporosis may be associated with encephalitis. This parasite can grow in brain endothelial cells without markedly damages, but it can modulate the cellular environment to promote its survival in the brain. In previous studies, we described that IFN-g decreased the parasite proliferation and down regulated nitric oxide (NO) production in astrocyte/microglia cultures. However,it remains unclear how glial cells respond to N. caninum in the presence of neurons. Therefore, we evaluated the effect of 300 IU/mL IFN-gamma or 1.0 mg/mL of LPS on infected rat neuron/glial co-cultures. After 72 h of infection, LPS did not affect the mitochondrial dehydrogenase activity. However, IFN-gamma decreased this parameter by 15.5 and 12.0% in uninfected and infected cells, respectively. The number of tachyzoites decreased 54.1 and 44.3% in cells stimulated with IFN-gamma and LPS, respectively. Infection or LPS treatment did not change NO production. On the other hand, IFN-gamma induced increased nitrite release in 55.7%, but the infection reverted this induction. IL-10 levels increased only in infected cultures (treated or not), meanwhile PGE2 release was improved in IFN-gamma/infected or LPS/infected cells. Although IFN-gamma significantly reduced the neurite length in uninfected cultures (42.64%; p < 0.001), this inflammatory cytokine reverted the impairment of neurite outgrowth induced by the infection (81.39%). The results suggest a neuroprotective potential response of glia to N. caninum infection under IFN-gamma stimulus. This observation contributes to understand the immune mediated mechanisms of neosporosis in central nervous system (CNS). |
| Databáze: | OpenAIRE |
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