Discrimination of the length of two remote binding sites by the spacer-linked DNA minor groove binders

Autor:	Shigeki Sasaki, Mikimasa Tanada
Rok vydání:	2006
Předmět:	Denticity Binding Sites Chemistry General Medicine DNA Surface Plasmon Resonance Ligands chemistry.chemical_compound Crystallography Duplex (building) Bisbenzimidazole Molecule Nucleic Acid Conformation A-DNA Binding site Surface plasmon resonance Dimerization Minor groove
Zdroj:	Nucleic acids symposium series (2004). (49)
ISSN:	1746-8272
Popis:	The aim of this study is to develop new recognition molecules for the DNA higher-ordered structure on the basis of new concept of DNA recognition mode. In order to bind two remote binding sites of DNA at the same time, we designed the new bidentate ligands that have two Hoechst 33258 units connected by a spacer. DNA binding affinity of the ligands was evaluated by measuring SPR (surface plasmon resonance). As a result, we have achieved discrimination of the length of two remote binding region of a DNA duplex.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbc8b1ddfd0a58ed6fb61495daefcf17 https://pubmed.ncbi.nlm.nih.gov/17150603 Zobrazit plný text záznamu