Diminished adhesion of CD4+ T cells from dialysis patients to extracellular matrix and its components fibronectin and laminin
| Autor: | Ze'ev Korzets, Y. A. Mekori, Mauro Rathaus, Ella Zeltzer, Rami Hershkoviz, Joelle Bernheim |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male T cell Integrin Extracellular matrix Immune system Antigen Cell–cell interaction medicine Cell Adhesion Humans Cell adhesion Aged Transplantation biology business.industry Integrin beta1 Cell Membrane Middle Aged Molecular biology Extracellular Matrix Fibronectins Renal Replacement Therapy medicine.anatomical_structure Nephrology Immunology biology.protein Female Laminin Cell activation business |
| Zdroj: | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 12(12) |
| ISSN: | 0931-0509 |
| Popis: | Background. Cell-mediated immunity is impaired in uraemia. The recognition and ensuing interactions of immune cells, such as CD4+ T lymphocytes, with adhesive glycoproteins of the extra-cellular matrix ( ECM ) are mediated by integrins of the b 1 subfamily. Introduction We have previously demonstrated that uraemic sera inhibit the proliferation and adhesion of normal An immunodeficient state is a recognized feature of CD4+ T cells to ECM components. In the present end-stage renal disease ( ESRD) manifested by prostudy, the adhesive capacity of CD4+ T lymphocytes longed tolerance to allografts [1], increased susceptibilof dialyzed patients (both haemodialysis [ HD] and ity to infections [2,3], an abnormally high incidence of continuous ambulatory peritoneal dialysis [CAPD] was neoplasia [4], anergy and a defective response to evaluated. vaccination [5,6 ]. The pathogenesis underlying this Methods. Adhesion of CD4+ T cells from dialysis impaired immunity is multifactorial, and concerns, patients to intact ECM and its immobilized moieties, among other things, T cell function, although the ratio fibronectin ( FN ) and laminin (LN ) was measured of CD 4 and CD 8 T cell subsets is within the normal following phorbol-12-myristate-13-acetate (PMA) range [7]. Uraemic serum markedly diminishes the stimulation. In addition, cell surface expression of b 1 proliferative response of normal T cells [8,9], sugintegrins ( VLA 4‐6) was determined by FACScan gesting the presence of circulating inhibitory subanalysis. stances. Uraemic T lymphocytes demonstrate an Results. Compared to normal cells, CD4+ T cells of impaired blastogenic response to mitogens and antidialysis patients demonstrated a significantly reduced gens [10]. The defective proliferation of T cells is adhesion to ECM, FN and LN (27‐28 vs 52‐55%, associated with a decreased production of interleukin-2 P |
| Databáze: | OpenAIRE |
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