Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line

Autor: Zainab J. Taqi, Majid S. Jabir, Dina A. Soliman, Usama I. Sahib, Ahmed Majeed Al-Shammari, Yaser Hassan Dewir, Salim Albukhaty, Ghassan M. Sulaiman, Ali A. Taha, Humaira Rizwana, Mona S. Alwahibi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
p53
linalool
Pharmaceutical Science
Metal Nanoparticles
Peptide
Apoptosis
02 engineering and technology
01 natural sciences
caspase-8
Antioxidants
chemistry.chemical_compound
SKOV-3
Linalool
Drug Discovery
Cytotoxicity
Original Research
chemistry.chemical_classification
Membrane Potential
Mitochondrial
Caspase 8
NF-kappa B
General Medicine
021001 nanoscience & nanotechnology
Glutathione
Up-Regulation
Gene Expression Regulation
Neoplastic
Protein Transport
Biochemistry
NF-κB translocation
CALNN
0210 nano-technology
Cell Nucleus Shape
Acyclic Monoterpenes
Biophysics
Down-Regulation
Bioengineering
Target peptide
Antineoplastic Agents
010402 general chemistry
Biomaterials
Cell Line
Tumor
Biomarkers
Tumor
Humans
Organic Chemistry
0104 chemical sciences
chemistry
Cell culture
gold nanoparticles
Cancer cell
Gold
Tumor Suppressor Protein p53
Peptides
DNA Damage
Mutagens
Zdroj: International Journal of Nanomedicine
ISSN: 1178-2013
1176-9114
Popis: Background Linalool is a monoterpene compound with various potential therapeutic applications in several medical fields. Previous studies have indicated the activity of linalool against cell lines; however, its high level of toxicity restricts its use. The aim of this study was to design and manufacture compounds with a novel structure that can be used for loading linalool, to reduce its toxicity and improve its reachable ability. Methods We synthesized and characterized a new molecule for loading linalool onto gold nanoparticles (GNPs) capped with glutathione and conjugated with a CALNN peptide. Linalool was loaded onto the GNPs via the reaction of the surface groups of both linalool and the GNPs. Moreover, the target peptide could be loaded onto the surface of the GNPs via a chemical reaction. The cytotoxic effects of linalool-GNP (LG) and linalool-GNP-CALNN peptide (LGC) conjugates against ovarian cancer cells were investigated, as were the possible mechanisms underlying the induction of apoptosis. Results Our findings illustrated the significant antiproliferative effect of LG and LGC on SKOV-3 cells. The cytotoxicity assay demonstrated that LG and LGC were selectively toxic in cancer cells and induced apoptosis by activating caspase-8, the p53 protein, and various proteins involved in apoptosis. The present data demonstrated that LG and LGC have a high therapeutic potential and should be given particular consideration as anticancer drug-delivery systems, as LG and LGC were remarkably more cytotoxic against a cancer cell line than were linalool and GNPs alone. Conclusion We concluded that LG and LGC are promising compounds that can be used for treating ovarian cancer (SKOV-3) cells via the induction of apoptosis through extrinsic and intrinsic pathways.
Databáze: OpenAIRE
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