The Binding of Thyroid Transcription Factor-1 and Hepatocyte Paraffin 1 to Mitochondrial Proteins in Hepatocytes
| Autor: | Hui Wu, Kumarasen Cooper, Jeannette Mitchell, Michele von Turkovich, Douglas J. Taatjes, Yijun Pang, Sharon L. Mount |
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| Rok vydání: | 2006 |
| Předmět: |
endocrine system
Carcinoma Hepatocellular Immunoelectron microscopy Thyroid Nuclear Factor 1 Thyroid Transcription Factor 1 Mitochondria Liver Mitochondrion Epitope Mitochondrial Proteins Western blot Biomarkers Tumor medicine Humans Binding site Microscopy Immunoelectron Binding Sites Staining and Labeling medicine.diagnostic_test Chemistry Thyroid Antibodies Monoclonal Nuclear Proteins General Medicine respiratory system Molecular biology Kidney Tubules medicine.anatomical_structure Hepatocyte Hepatocytes Urothelium Transcription Factors |
| Zdroj: | American Journal of Clinical Pathology. 125:722-726 |
| ISSN: | 1943-7722 0002-9173 |
| Popis: | We studied the antibodies hepatocyte paraffin 1 (Hep Par 1) and thyroid transcription factor-1 (TTF-1; clone 8G7G3/1) in normal human liver tissue with immunoelectron microscopy using renal tubules as control samples. TTF-1 (8G7G3/1) and Hep Par 1 bound exclusively to the mitochondria of hepatocytes but not to those of the renal tubular epithelium. Both antibodies labeled mitochondria in a similar pattern and density. These findings confirm that the binding site of Hep Par 1 in hepatocytes is mitochondrial. The specific binding of TTF-1 (8G7G3/1) in hepatocyte mitochondria suggests its potential usefulness for identifying hepatocellular carcinoma. Western blot analysis with cellular proteins extracted from normal human liver and thyroid tissue demonstrated that Hep Par 1 and TTF-1 (8G7G3/1) bound to a protein band of approximately 150 kd in liver cells, with TTF-1 (8G7G3/1) showing less affinity. It is likely that different epitopes to Hep Par 1 and TTF-1 (8G7G3/1) share the same protein molecule in hepatocyte mitochondria. |
| Databáze: | OpenAIRE |
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