An AP2/ERF transcription factor ERF139 coordinates xylem cell expansion and secondary cell wall deposition
| Autor: | Jaakko Kangasjärvi, Jorma Vahala, Judith Felten, Carolin Seyfferth, Thomas Vain, Michaela Eder, Nicolas Delhomme, Sacha Escamez, Kamil Antos, Hannele Tuominen, Bernard Wessels |
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| Přispěvatelé: | Plant Biology, Organismal and Evolutionary Biology Research Programme, Viikki Plant Science Centre (ViPS), Plant ROS-Signalling |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Programmed cell death Physiology Secondary growth Repressor lignin Plant Science 01 natural sciences xylem development Transcriptome 03 medical and health sciences Cell expansion secondary growth X-Ray Diffraction hybrid aspen Cell Wall Gene Expression Regulation Plant Xylem Plant Cells Transcriptional regulation Ethylene Response Factor (ERF) Transcription factor 1183 Plant biology microbiology virology Plant Proteins Chemistry fungi food and beverages Ethylenes Plants Genetically Modified Wood Cell biology 030104 developmental biology Populus Transcription Factor AP-2 Secondary cell wall Signal Transduction 010606 plant biology & botany |
| Popis: | Differentiation of xylem elements involves cell expansion, secondary cell wall deposition and programmed cell death. Transitions between these phases require strict spatiotemporal control. The function of Populus ERF139 (Potri.013G101100) in xylem differentiation was characterized in transgenic overexpression and dominant repressor lines of ERF139 in hybrid aspen (Populus tremula x tremuloides). Xylem properties, secondary cell wall (SCW) chemistry and downstream targets were analyzed in both types of transgenic trees using microscopy techniques, FT-IR, pyrolysis-GC/MS, wet chemistry methods and RNA sequencing. Opposite phenotypes were observed in the secondary xylem vessel sizes and SCW chemistry in the two different types of transgenic trees, supporting the function of ERF139 in suppressing the radial expansion of vessel elements and stimulating accumulation of guaiacyl-type lignin and possibly also xylan. Comparative transcriptomics identified genes related to SCW biosynthesis (LAC5, LBD15, MYB86) and salt and drought stress responsive genes (ANAC002, ABA1) as potential direct targets of ERF139. The phenotypes of the transgenic trees and the stem expression profiles of ERF139 potential target genes support the role of ERF139 as a transcriptional regulator of xylem cell expansion and SCW formation, possibly in response to osmotic changes of the cells. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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