Bacterial lipopolysaccharide inhibits influenza virus infection of human macrophages and the consequent induction of CD8+ T cell immunity
| Autor: | Short, Kirsty R, Vissers, Marloes, de Kleijn, Stan, Zomer, Aldert L, Kedzierska, Katherine, Grant, Emma, Reading, Patrick C, Hermans, Peter W M, Ferwerda, Gerben, Diavatopoulos, Dimitri A, LS Klinisch Onderzoek Wagenaar |
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| Přispěvatelé: | LS Klinisch Onderzoek Wagenaar |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Antigen presentation lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] Other Research Radboud Institute for Molecular Life Sciences [Radboudumc 0] Biology CD8-Positive T-Lymphocytes Virus Replication Virus Microbiology Madin Darby Canine Kidney Cells 03 medical and health sciences Interferon-gamma Immune system Dogs Antigen Interferon medicine Immunology and Allergy Cytotoxic T cell Animals Humans Cells Cultured 030304 developmental biology Oligonucleotide Array Sequence Analysis 0303 health sciences Antigen Presentation Bacterial disease 030306 microbiology Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Macrophages Interferon-beta Acquired immune system Flow Cytometry Coculture Techniques 3. Good health Influenza A virus Immunology Host-Pathogen Interactions Transcriptome medicine.drug HeLa Cells Research Article |
| Zdroj: | Journal of Innate Immunity, 6(2), 129. S. Karger AG Journal of Innate Immunity, 6, 2, pp. 129-39 Journal of Innate Immunity, 6, 129-39 |
| ISSN: | 1662-811X |
| Popis: | Contains fulltext : 127685.pdf (Publisher’s version ) (Open Access) It is well established that infection with influenza A virus (IAV) facilitates secondary bacterial disease. However, there is a growing body of evidence that the microbial context in which IAV infection occurs can affect both innate and adaptive responses to the virus. To date, these studies have been restricted to murine models of disease and the relevance of these findings in primary human cells remains to be elucidated. Here, we show that pre-stimulation of primary human monocyte-derived macrophages (MDMs) with the bacterial ligand lipopolysaccharide (LPS) reduces the ability of IAV to infect these cells. The inhibition of IAV infection was associated with a reduced transcription of viral RNA and the ability of LPS to induce an anti-viral/type I interferon response in human MDMs. We demonstrated that this reduced rate of viral infection is associated with a reduced ability to present a model antigen to autologous CD8+ T cells. Taken together, these data provide the first evidence that exposure to bacterial ligands like LPS can play an important role in modulating the immune response of primary human immune cells towards IAV infection, which may then have important consequences for the development of the host's adaptive immune response. |
| Databáze: | OpenAIRE |
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