SHORT IN VITRO HALF-LIFE OF THYMOPOIETIN 32-36 PENTAPEPTIDE IN HUMAN PLASMA
| Autor: | Mark Chasin, John Tischio, James E. Patrick, Howard Weintraub, Gideon Goldstein |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Chemical Phenomena Thymopoietins Biochemistry Pentapeptide repeat In vivo medicine Humans Thymopentin Amino Acid Sequence Thymopoietin chemistry.chemical_classification Chromatography biology Chemistry Half-life Biological activity Peptide Fragments In vitro Amino acid Thymus Hormones biology.protein Chromatography Thin Layer Half-Life medicine.drug |
| Zdroj: | International Journal of Peptide and Protein Research. 14:479-484 |
| ISSN: | 0367-8377 |
| Popis: | Thymopoietin32--36 (TP5) is a synthetic pentapeptide that has the biological activity of its parent molecule, the 49 amino acid thymic hormone thymopoietin. Tritiated thymopoietin32--36 (3 /-TP5) was prepared by reductive tritiation of dibromotyrosyl-TP5. The stability of 3 H-TP5 in human plasma was studied by analyzing samples by thin-layer chromatography at different time points and quantitating the radioactivity associated with TP5 (Arg-Lys-Asp-Val-Tyr) and its tyrosyl-containing breakdown products (Lys-Asp-Val-Tyr, Asp-Val-Tyr, Val-Tyr, Tyr). In plasma (but not in saline) the pentapeptide was rapidly degraded (apparent t1/2 approximately 30 seconds) with the corresponding appearance of radioactivity associated with the other tyrosyl-containing reference compounds. These data imply that the pentapeptide, which is active in vivo, may rapidly trigger changes in responsive cells; sustained circulating levels may not be required for activity. |
| Databáze: | OpenAIRE |
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