Dynamic phosphorylation of VE-cadherin Y685 throughout mouse estrous cycle in ovary and uterus
| Autor: | Soumalamaya Bama, Isabelle Vilgrain, Adama Sidibé, Laurence Bouillet, Helena Polena, Tiphaine Mannic, Nicolas Chaumontel, Philippe Huber, Danielle Gulino-Debrac, Jeremy Razanajatovo, Irene Marechal |
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| Přispěvatelé: | Université Grenoble Alpes - Institut d'Administration des Entreprises (UGA IAE), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire de développement et vieillissement de l'endothélium, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie vasculaire : interactions cellulaires, signalisation et vieillissement, Clinique de médecine interne, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Time Factors
Gonadotropins Equine Physiology Angiogenesis [SDV]Life Sciences [q-bio] Chorionic Gonadotropin chemistry.chemical_compound 0302 clinical medicine Phosphorylation reproductive and urinary physiology ComputingMilieux_MISCELLANEOUS 0303 health sciences Cadherins [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Vascular endothelial growth factor src-Family Kinases medicine.anatomical_structure 030220 oncology & carcinogenesis Female Cardiology and Cardiovascular Medicine Tyrosine kinase hormones hormone substitutes and hormone antagonists Proto-oncogene tyrosine-protein kinase Src endocrine system medicine.medical_specialty Estrous Cycle Ovary [SDV.BC]Life Sciences [q-bio]/Cellular Biology Vascular Remodeling Biology 03 medical and health sciences Antigens CD Physiology (medical) Internal medicine medicine Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] 030304 developmental biology Estrous cycle urogenital system Uterus Tyrosine phosphorylation Mice Inbred C57BL Endocrinology chemistry Tyrosine Reports |
| Zdroj: | AJP-Heart and Circulatory Physiology AJP-Heart and Circulatory Physiology, 2014, 307 (3), pp.H448-H454 AJP-Heart and Circulatory Physiology, American Physiological Society, 2014, 307 (3), pp.H448-H454 |
| ISSN: | 0363-6135 1522-1539 |
| Popis: | We previously reported that vascular endothelial growth factor induced vascular endothelial (VE)-cadherin tyrosine phosphorylation at Y685 in a Src-dependent manner in vitro. Here, we studied the occurrence of Y685 phosphorylation in vivo in the female reproductive tract because it is a unique model of physiological vascular remodeling dependent on vascular endothelial growth factor. We first developed and characterized an anti-phospho-specific antibody against the site Y685 of VE-cadherin to monitor VE-cadherin phosphorylation along the four phases of mouse estrous cycle, termed proestrus, estrus, metestrus, and diestrus. A dynamic profile of tyrosine phosphorylated proteins was observed in both uterus and ovary throughout mouse estrous cycle, including kinase Src, which was found highly active at the estrus phase. The extent of tyrosine phosphorylated VE-cadherin was low at proestrus but strongly increased at estrus and returned to baseline at metestrus and diestrus, suggesting a potent hormonal regulation of this specific process. Indeed, C57Bl/6 female mice treatment with pregnant mare serum gonadotropin and human chorionic gonadotropin confirmed a significant increase in phosphoY685-VE-cadherin compared with that in untreated mice. These results demonstrate that VE-cadherin tyrosine phosphorylation at Y685 is a physiological and hormonally regulated process in female reproductive organs. In addition, this process was concomitant with the early steps of vascular remodeling taking place at estrus stage, suggesting that phosphoY685-VE-cadherin is a biomarker of endothelial cell activation in vivo. |
| Databáze: | OpenAIRE |
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