Comparison of PF4/heparin ELISA assay with the 14C-serotonin release assay in the diagnosis of heparin-induced thrombocytopenia
| Autor: | Douglas B. Cines, Carol Reynolds, Anne Tomaski, Jean Amiral, Gowthami M. Arepally, Abbas F. Jawad, Mortimer Poncz |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
medicine.medical_specialty Serotonin medicine.drug_class Enzyme-Linked Immunosorbent Assay Platelet Factor 4 Gastroenterology Sensitivity and Specificity Antibodies Internal medicine Heparin-induced thrombocytopenia medicine Humans Platelet Carbon Radioisotopes biology business.industry Heparin Anticoagulant General Medicine Elisa assay medicine.disease Thrombocytopenia Immunology Toxicity biology.protein Biological Assay Antibody business Platelet factor 4 medicine.drug |
| Zdroj: | American journal of clinical pathology. 104(6) |
| ISSN: | 0002-9173 |
| Popis: | The diagnosis of heparin-induced thrombocytopenia (HIT) may be affirmed by demonstrating heparin-dependent anti-platelet antibodies using the 14C-serotonin release assay (SRA). In this study, results of the SRA was compared with the recently described platelet factor 4 (PF4)/heparin enzyme-linked immunosorbent assay (ELISA). Compared with the SRA, the sensitivity and specificity of a PF4/heparin ELISA was 87% and 92%, respectively, using an assay developed in our laboratory; and 90% and 98%, respectively, using a commercially developed kit (Diagnostica Stago, Asnieres, France). However, antibodies to PF4/heparin were also detected in up to 8% of patients whose plasma was negative by SRA, and 23% of patients receiving heparin who were not thrombocytopenic. These data indicate that results obtained with the PF4/heparin ELISA and the SRA are generally in accord in patients with a clinical diagnosis of HIT. However, discrepant results occur in approximately 20% of cases because of the greater sensitivity of ELISA and the possible involvement of other heparin-binding proteins. The fact that each assay contributes independent information in some cases must be considered in the sequence of test performance and in providing consultation to the practicing hematologist. |
| Databáze: | OpenAIRE |
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