Calcium, manganese and hepatic gluconeogenesis
| Autor: | Naomi Friedmann, Howard Rasmussen |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Calcium Isotopes
Male medicine.medical_specialty Basal rate Glycogenolysis Biophysics chemistry.chemical_element Iodoacetates Stimulation In Vitro Techniques Calcium Biochemistry Glucagon Isotopes of calcium Oxygen Consumption Tetracaine Internal medicine Cyclic AMP medicine Animals Pyruvates Molecular Biology Radioisotopes Calcium metabolism Manganese Gluconeogenesis Fasting Stimulation Chemical Liver Glycogen Rats Cold Temperature Perfusion Endocrinology Liver chemistry Strontium Depression Chemical Lactates Dinitrophenols |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 222:41-52 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(70)90349-1 |
| Popis: | In the isolated perfused rat liver, administration of either glucagon or 3′,5′-AMP is followed by an efflux of Ca 2+ and enhanced glucose formation from lactate or pyruvate. The release of Ca 2+ precedes the increase in glucose production. The Ca 2+ is released from a pool which also accumulates Mn 2+ and Sr 2+ . Addition of either iodoacetate or 2,4-dinitrophenol leads to an increase in total Ca 2+ uptake by the liver, as measured with 45 Ca 2+ , but a decreased accumulation of 45 Ca 2+ into the cyclic nucleotide-sensitive pool. Changes in the concentration of Ca 2+ in the perfusate had little effect upon gluconeogenesis, but the addition of Mn 2+ stimulated gluconeogenesis without altering 3′,5′-AMP concentration. Perfusion of the liver with 1· 10 −4 M tetracaine did not greatly alter the basal rate of glucose formation or O 2 consumption, but did inhibit either 3′,5′-AMP or glucagon-induced gluconegenesis or glycogenolysis, and nucleotide-induced increase in Ca 2+ efflux. Tetracaine did not block the glucagon-induced increase in 3′,5′, AMP. |
| Databáze: | OpenAIRE |
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