Effects of genome duplication on phenotypes and industrial applications of Saccharomyces cerevisiae strains
| Autor: | Ya-Hong Fang, Gao Kehui, Dao-Qiong Zheng, Yang Sui, Ke Zhang, Xuechang Wu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins 030106 microbiology Saccharomyces cerevisiae Gene Expression Biology Cell morphology Real-Time Polymerase Chain Reaction Applied Microbiology and Biotechnology Genome Polyploidy 03 medical and health sciences Industrial Microbiology Stress Physiological Ergosterol Gene Duplication Gene duplication Gene Genetics Ethanol Strain (biology) Trehalose General Medicine biology.organism_classification Yeast Phenotype Fermentation Ploidy Genome Fungal Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 101(13) |
| ISSN: | 1432-0614 |
| Popis: | Polyploidy is common in Saccharomyces cerevisiae strains, but the physiological and phenotypic effects of ploidy changes have not been fully clarified. Here, isogenic diploid, triploid, and tetraploid S. cerevisiae strains were constructed from a haploid strain, CEN.PK2-1C. Stress tolerance and ethanol fermentation performance of the four euploid strains were compared. Each euploid strain had strengths and weaknesses in tolerance to certain stressors, and no single strain was tolerant of all stressors. The diploid had higher ethanol production than the other strains in normal fermentation medium, while the triploid strain showed the fastest fermentation rate in the presence of inhibitors found in lignocellulosic hydrolysate. Physiological determination revealed diverse physiological attributes, such as trehalose, ergosterol, glutathione, and anti-oxidative enzymes among the strains. Our analyses suggest that both ploidy parity and number of chromosome sets contribute to changes in physiological status. Using qRT-PCR, different expression patterns of genes involved in the regulation of cell morphology and the biosynthesis of key physiological attributes among strains were determined. Our data provide novel insights into the multiple effects of genome duplication on yeast cells and are a useful reference for breeding excellent strains used in specific industrial applications. |
| Databáze: | OpenAIRE |
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