Foxi transcription factors promote pharyngeal arch development by regulating formation of FGF signaling centers

Autor: Renée K. Edlund, Andrew K. Groves, Bruce B. Riley, Takahiro Ohyama, Husniye Kantarci
Rok vydání: 2014
Předmět:
Embryo
Nonmammalian
Apoptosis
Ectoderm
Animals
Genetically Modified
Mice
0302 clinical medicine
Cranial neural crest
Cell Movement
FGF
Pharyngeal arch
In Situ Hybridization
Zebrafish
Mice
Knockout
0303 health sciences
Endoderm
Gene Expression Regulation
Developmental
Neural crest
Forkhead Transcription Factors
Anatomy
medicine.anatomical_structure
Neural Crest
Gene Knockdown Techniques
embryonic structures
Craniofacial development
Signal Transduction
Mice
129 Strain
animal structures
Fibroblast Growth Factor 8
Ectomesenchyme
Fibroblast Growth Factor 3
Branchial arch
Biology
Article
03 medical and health sciences
medicine
Animals
Molecular Biology
Body Patterning
030304 developmental biology
Cell Biology
Zebrafish Proteins
Embryo
Mammalian
Fibroblast Growth Factors
body regions
Branchial Region
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Developmental Biology. 390(1):1-13
ISSN: 0012-1606
DOI: 10.1016/j.ydbio.2014.03.004
Popis: The bones of the vertebrate face develop from transient embryonic branchial arches that are populated by cranial neural crest cells. We have characterized a mouse mutant for the Forkhead family transcription factor Foxi3, which is expressed in branchial ectoderm and endoderm. Foxi3 mutant mice are not viable and display severe branchial arch-derived facial skeleton defects, including absence of all but the most distal tip of the mandible and complete absence of the inner, middle and external ear structures. Although cranial neural crest cells of Foxi3 mutants are able to migrate, populate the branchial arches, and display some elements of correct proximo-distal patterning, they succumb to apoptosis from embryonic day 9.75 onwards. We show this cell death correlates with a delay in expression of Fgf8 in branchial arch ectoderm and a failure of neural crest cells in the arches to express FGF-responsive genes. Zebrafish foxi1 is also expressed in branchial arch ectoderm and endoderm, and morpholino knock-down of foxi1 also causes apoptosis of neural crest in the branchial arches. We show that heat shock induction of fgf3 in zebrafish arch tissue can rescue cell death in foxi1 morphants. Our results suggest that Foxi3 may play a role in the establishment of signaling centers in the branchial arches that are required for neural crest survival, patterning and the subsequent development of branchial arch derivatives.
Databáze: OpenAIRE
Externí odkaz: