Aucubin ameliorates the LPS-induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF-κB and activating the Keap1/Nrf2 signalling pathway
| Autor: | Rongfeng Cao, Wenru Tian, Yanni Feng, Feng Gao, Huatao Li, Kaiqiang Fu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
NF-E2-Related Factor 2 Iridoid Glucosides Anti-Inflammatory Agents Inflammation Apoptosis Flow cytometry 03 medical and health sciences chemistry.chemical_compound Endometrium 0302 clinical medicine Endocrinology medicine Animals Cells Cultured 030219 obstetrics & reproductive medicine Kelch-Like ECH-Associated Protein 1 medicine.diagnostic_test 0402 animal and dairy science NF-kappa B NF-κB Epithelial Cells 04 agricultural and veterinary sciences 040201 dairy & animal science KEAP1 Molecular biology Hedgehog signaling pathway Blot chemistry Phosphorylation Animal Science and Zoology Cattle Female medicine.symptom Biotechnology Signal Transduction |
| Zdroj: | Reproduction in domestic animals = ZuchthygieneReferences. 56(7) |
| ISSN: | 1439-0531 |
| Popis: | Cows are susceptible to pathogenic bacterial infection after pregnancy, leading to inflammation of the endometrium. Aucubin (AU) has been proven to exhibit highly effective anti-inflammatory activity, but its ability to protect against endometritis in dairy cows remains unclear. Therefore, the goal of the present study was to evaluate the protective effect of AU on the LPS-induced inflammatory response of bovine endometrial epithelial cells (BEECs). After pre-treating BEECs with AU (10, 20 and 50 μM) for 6 hr, the cells were stimulated with LPS for 3 hr. Subsequently, BEECs apoptosis was analysed by flow cytometry, the expression of pro-inflammatory cytokine mRNA was detected by qRT-PCR, and changes in NF-κB and Keap1/Nrf2 signalling were analysed by western blotting and immunofluorescence analyses. The results showed that AU can reduce TNF-α, IL-1β, IL-6, COX-2 and iNOS mRNA expression in BEECs and reduce cell apoptosis. Furthermore, AU significantly reduced the level of NF-κB p65 and IκB phosphorylation and inhibited the nuclear translocation of NF-κB p65. AU also activated the Keap1/Nrf2 pathway, promoting the nuclear transfer of Nrf2 and increasing Keap1, Nrf2, HO-1 and NQO1 mRNA and protein levels. Taken together, these results indicate that AU ameliorates the LPS-induced inflammatory response by inhibiting NF-κB and activating the Keap1/Nrf2 signalling pathway, which has a protective effect on BEECs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text