A conserved odorant binding protein is required for essential amino acid detection in Drosophila
| Autor: | Teiichi Tanimura, Loïc Briand, Isabelle Chauvel, Nicolas Poirier, Karen Rihani, Thomas Delompré, Jean-François Ferveur, Fabrice Neiers, Stéphane Fraichard |
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| Přispěvatelé: | Ferveur, Jean-François, Briand, Loïc, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), Université de Nagoya - Nagoya University (.) (NU), Leibniz Institute for Neurobiology, Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Carrier proteins [SDV]Life Sciences [q-bio] Mutant Medicine (miscellaneous) Fluorescent Antibody Technique Gene Expression Receptors Odorant General Biochemistry Genetics and Molecular Biology Article law.invention Evolution Molecular 03 medical and health sciences 0302 clinical medicine law Biologie animale Animals Food and Nutrition [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acids lcsh:QH301-705.5 Drosophila Sensillum Essential amino acid Conserved Sequence chemistry.chemical_classification Animal biology biology fungi biology.organism_classification 3. Good health Amino acid Electrophysiological Phenomena 030104 developmental biology lcsh:Biology (General) chemistry Biochemistry Feeding behaviour Alimentation et Nutrition Odorant-binding protein biology.protein Recombinant DNA melanogaster preference family reveals receptor bitter taste organization neurons pheromone-binding ligand-binding Amino Acids Essential Drosophila melanogaster General Agricultural and Biological Sciences 030217 neurology & neurosurgery |
| Zdroj: | Communications biology, 2:425 Communications Biology 1 (2), 425. (2019) Communications Biology Communications Biology, Nature Publishing Group, 2019, 2 (1), pp.425. ⟨10.1038/s42003-019-0673-2⟩ Communications Biology, Vol 2, Iss 1, Pp 1-10 (2019) |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-019-0673-2⟩ |
| Popis: | Animals need to detect in the food essential amino acids that they cannot synthesize. We found that the odorant binding protein OBP19b, which is highly expressed in Drosophila melanogaster taste sensilla, is necessary for the detection of several amino acids including the essential l-phenylalanine. The recombinant OBP19b protein was produced and characterized for its binding properties: it stereoselectively binds to several amino acids. Using a feeding-choice assay, we found that OBP19b is necessary for detecting l-phenylalanine and l-glutamine, but not l-alanine or D-phenylalanine. We mapped the cells expressing OBP19b and compared the electrophysiological responses of a single taste sensillum to several amino acids: OBP19b mutant flies showed a reduced response compared to control flies when tested to preferred amino acids, but not to the other ones. OBP19b is well conserved in phylogenetically distant species suggesting that this protein is necessary for detection of specific amino acids in insects. Karen Rihani et al. demonstrate that fruit flies need an odorant-binding protein OBP19b, which is highly expressed in taste sensilla, to prefer select amino acids such as essential l-phenylalanine. This study provides insights into the mechanisms by which insects ensure their dietary intake of essential amino acids. |
| Databáze: | OpenAIRE |
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