Steady-state transcript levels of cytochrome c oxidase genes during human myogenesis indicate subunit switching of subunit VIa and co-expression of subunit VIIa isoforms
| Autor: | H Devries, Nh Herzberg, Jw Taanman, C Vandenbogert, Pa Bolhuis |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Gene isoform
Adult Male Cytochrome Transcription Genetic Protein subunit Electron Transport Complex IV RNA Ribosomal 16S Cytochrome c oxidase Humans Northern blot RNA Messenger Molecular Biology Cells Cultured Adenosine Triphosphatases biology ATP synthase Myogenesis Muscles Molecular biology Mitochondria Muscle Isoenzymes biology.protein Molecular Medicine Female sense organs ATP synthase alpha/beta subunits |
| Zdroj: | Biochimica et biophysica acta. 1139(1-2) |
| ISSN: | 0006-3002 |
| Popis: | Steady-state levels of the mitochondrial rRNAs, of mRNAs for mitochondrially and nuclear-encoded subunits of cytochrome c oxidase and for the beta subunit of ATP synthase were assessed by Northern blot hybridizations during the in vitro differentiation of human myoblasts. Transcript levels of the so-called liver-type form of subunit VIa of cytochrome c oxidase diminished during the course of differentiation, while transcription of the so-called heart-type form was induced. Transcripts for the liver-type form and for the heart-type form of subunit VIIa of cytochrome c oxidase were detected in all myogenic cultures; the levels of the heart-type form progressively increased during the course of differentiation. The levels of the other transcripts studied did not change substantially. The results suggest subunit switching of subunit VIa and co-expression of subunit VIIa isoforms during myogenesis. The differential changes in mRNA levels of the heart-type subunits VIa and VIIa and the differential changes in mRNA levels of the liver-type subunits VIa and VIIa demonstrate that different transcriptional regulation mechanisms are present for both heart-type genes as well as for both liver-type genes. |
| Databáze: | OpenAIRE |
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