Bufalin induced apoptosis in SCC-4 human tongue cancer cells by decreasing Bcl-2 and increasing Bax expression via the mitochondria-dependent pathway

Autor: Ming Jen Fan, Fu Shin Chueh, Rick Sai-Chuen Wu, Han‑Yu Chou, Wen Wen Huang, Yi Shih Ma, Jing Gung Chung, Ching Lung Liao, Yung Lin Chu
Rok vydání: 2017
Předmět:
0301 basic medicine
Cancer Research
Cell
Apoptosis
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
Cytotoxic T cell
bcl-2-Associated X Protein
Membrane Potential
Mitochondrial
medicine.diagnostic_test
DNA ladder
Articles
Cell cycle
Chromatin
Mitochondria
Tongue Neoplasms
Cell biology
G2 Phase Cell Cycle Checkpoints
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
Oncology
030220 oncology & carcinogenesis
SCC-4 cells
Molecular Medicine
Signal Transduction
Cell Survival
Antineoplastic Agents
DNA Fragmentation
Biology
Nitric Oxide
Flow cytometry
03 medical and health sciences
Cell Line
Tumor
Genetics
medicine
Humans
DAPI
Molecular Biology
Bufalin
Bufanolides
030104 developmental biology
chemistry
Cancer cell
Apoptosis Regulatory Proteins
Reactive Oxygen Species
bufalin
DNA Damage
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
DOI: 10.3892/mmr.2017.7651
Popis: The aim of the present study was to investigate the cytotoxic effects of bufalin on SCC-4 human tongue cancer cells. Cell morphological changes and viability were examined using phase contrast microscopy and flow cytometry, respectively. The results indicated that bufalin induced morphological changes and reduced total viable cells. Apoptotic cell death was analyzed by DAPI staining and DNA gel electrophoresis; the results revealed that bufalin induced cell apoptosis. Levels of reactive oxygen species (ROS), Ca2+, nitric oxide (NO) and mitochondrial membrane potential (ΔΨm) were measured by flow cytometry, and bufalin was observed to increase Ca2+ and NO production, decrease the ΔΨm and reduce ROS production in SCC-4 cells. In addition, western blotting was performed to detect apoptosis-associated protein expression. The results demonstrated that bufalin reduced the expression of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and increased the expression of the pro-apoptotic protein, Bcl-2-associated X protein. However, bufalin treatment also increased the expression of other apoptosis-associated proteins such as apoptosis-inducing factor and endonuclease G in SCC-4 cells. Based on these findings, bufalin may induce apoptotic cell death via mitochondria-dependent pathways in human tongue cancer SCC-4 cells.
Databáze: OpenAIRE
Externí odkaz: