Identification of cDNA clones encoding HMG 2, a major protein of the mexican axolotl hydrocortisone-sensitive thymocytes
| Autor: | Arnaud De Guerra, Jacques Charlemagne, Julien S. Fellah, Francoise Guillet |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary HMG-box T-Lymphocytes Immunology Molecular Sequence Data Sequence Homology Spleen Polymerase Chain Reaction chemistry.chemical_compound Axolotl Complementary DNA medicine Animals Amino Acid Sequence Cloning Molecular Gene Gene Library biology Base Sequence High Mobility Group Proteins biology.organism_classification Molecular biology Ambystoma mexicanum medicine.anatomical_structure chemistry Cytoplasm biology.protein Antibody DNA Developmental Biology |
| Zdroj: | Developmental and comparative immunology. 19(5) |
| ISSN: | 0145-305X |
| Popis: | We have identified and analyzed cDNA clones encoding a major 26 kDa protein of the HMG 1–2 family which is abundant in the cytoplasm and nucleus of axolotl hydrocortisone-sensitive thymocytes. The axolotl HMG2 protein is very similar to proteins belonging to the HMG1–2 family, from teleost fish to mammals. All the molecular features of the HMG 1–2 proteins are conserved, including the high proportion of basic and aromatic residues, and the characteristic acidic C-terminus tail. The 3′-untranslated region of the HMG2 axolotl cDNA is also similar to the avian and mammalian HMG2 3 ' -UT sequences, suggesting that some selective events have acted at the DNA level to conserve this region, which could be important in the differential expression of the HMG1 and HMG2 genes. The axolotl HMG2 protein contains the two well conserved HMG boxes which are thought to be the DNA-binding domains of the molecule. Axolotl thymocytes and spleen cells contain almost identical amounts of HMG2 mRNAs but HMG2 polypeptide is undetectable in spleen cells using anti-26 kDa antibodies. The reason for the accumulation of HMG1–2 molecules in vertebrate hydrocortisone-sensitive thymocytes is discussed, as well as their possible role in apoptosis. |
| Databáze: | OpenAIRE |
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