Genetic Analyses of the FRNK Motif Function of Turnip mosaic virus Uncover Multiple and Potentially Interactive Pathways of Cross-Protection
| Autor: | Chan-Pin Lin, Shyi-Dong Yeh, Shih-Shun Lin, Yi-Jung Kung, Chin-Chih Chen, Pin-Chun Lin, Syuan-Fei Hong, Hui-Wen Wu, Yu-Hsin Huang, Nam-Hai Chua, Li-Yu Liu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Ribonuclease III
Physiology Cross Protection Amino Acid Motifs Potyvirus Arabidopsis Nicotiana benthamiana Cell Cycle Proteins Models Biological Virus Viral Proteins Gene Expression Regulation Plant RNA interference Tobacco Gene silencing Turnip mosaic virus Plant Diseases Genetics biology Arabidopsis Proteins Sequence Analysis RNA High-Throughput Nucleotide Sequencing RNA General Medicine Plants Genetically Modified biology.organism_classification Plant Leaves Cysteine Endopeptidases MicroRNAs RNA silencing Amino Acid Substitution Seedlings Mutation RNA Interference Salicylic Acid Agronomy and Crop Science |
| Zdroj: | Molecular Plant-Microbe Interactions®. 27:944-955 |
| ISSN: | 1943-7706 0894-0282 |
| Popis: | Cross-protection triggered by a mild strain of virus acts as a prophylaxis to prevent subsequent infections by related viruses in plants; however, the underling mechanisms are not fully understood. Through mutagenesis, we isolated a mutant strain of Turnip mosaic virus (TuMV), named Tu-GK, that contains an Arg182Lys substitution in helper component-proteinase (HC-ProK) that confers complete cross-protection against infection by a severe strain of TuMV in Nicotiana benthamiana, Arabidopsis thaliana Col-0, and the Arabidopsis dcl2-4/dcl4-1 double mutant defective in DICER-like ribonuclease (DCL)2/DCL4-mediated silencing. Our analyses showed that HC-ProK loses the ability to interfere with microRNA pathways, although it retains a partial capability for RNA silencing suppression triggered by DCL. We further showed that Tu-GK infection triggers strong salicylic acid (SA)-dependent and SA-independent innate immunity responses. Our data suggest that DCL2/4-dependent and –independent RNA silencing pathways are involved, and may crosstalk with basal innate immunity pathways, in host defense and in cross-protection. |
| Databáze: | OpenAIRE |
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