THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS
| Autor: | David G. McConnell |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Chemical Phenomena
Respiratory chain In Vitro Techniques Biology Mitochondrion Reductase Article Retina Electron Transport Complex IV chemistry.chemical_compound Animals Adenosine Triphosphatases Differential centrifugation Histocytochemistry Retinal Cell Biology Molecular biology Mitochondria Chemistry Microscopy Electron Mitochondrial respiratory chain Biochemistry chemistry Glucose-6-Phosphatase Microsome Cattle Oxidoreductases Polarography Subcellular Fractions |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Bovine retinal outer segment fragments were isolated by density gradient centrifugation in a high centrifugal field. Assays of the final preparation for enzymes of the mitochondrial respiratory chain indicated mitochondrial contamination not in excess of 1 per cent. Glucose-6-phosphatase and TPNH-cytochrome c reductase activities, presumably diagnostic for microsomes, were also absent. Electron micrographs did not disclose the presence of significant numbers of particles other than fragments of the outer segment discs. The red fragments were characterized by an ascorbate-oxidizing system and a high lipid content. |
| Databáze: | OpenAIRE |
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