Correction draft: RNA-Mediated Thermoregulation of Iron-Acquisition Genes in Shigella dysenteriae and Pathogenic Escherichia coli
| Autor: | Jens Kortmann, Erin R. Murphy, Franz Narberhaus, Andrew B. Kouse, Francesco Righetti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Transcription
Genetic DNA Mutational Analysis lcsh:Medicine medicine.disease_cause Biochemistry RNA thermometer chemistry.chemical_compound Molecular cell biology Genes Reporter RNA structure Promoter Regions Genetic lcsh:Science Heme Genetics Multidisciplinary Temperature Bacterial Pathogens Nucleic acids Host-Pathogen Interaction RNA Bacterial Medical Microbiology Research Article Shigella dysenteriae Iron Green Fluorescent Proteins Molecular Sequence Data Environment Biology Microbiology Models Biological Molecular Genetics Bacterial Proteins Escherichia coli medicine Humans Gene Regulation Computer Simulation RNA Messenger Microbial Pathogens Gene Gram Positive Base Sequence lcsh:R Correction RNA Bacteriology Pathogenic bacteria Gene Expression Regulation Bacterial biology.organism_classification Heme A chemistry Genes Bacterial Protein Biosynthesis Mutagenesis Site-Directed Nucleic Acid Conformation lcsh:Q 5' Untranslated Regions |
| Zdroj: | PLoS ONE, Vol 8, Iss 5, p e63781 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The initiation, progression and transmission of most bacterial infections is dependent upon the ability of the invading pathogen to acquire iron from each of the varied environments encountered during the course of a natural infection. In total, 95% of iron within the human body is complexed within heme, making heme a potentially rich source of host-associated nutrient iron for invading bacteria. As heme is encountered only within the host, pathogenic bacteria often regulate synthesis of heme utilization factors such that production is maximal under host-associated environmental conditions. This study examines the regulated production of ShuA, an outer-membrane receptor required for the utilization of heme as a source of nutrient iron by Shigella dysenteriae, a pathogenic bacterium that causes severe diarrheal diseases in humans. Specifically, the impact of the distinct environmental temperatures encountered during infection within a host (37°C) and transmission between hosts (25°C) on shuA expression is investigated. We show that shuA expression is subject to temperature-dependent post-transcriptional regulation resulting in increased ShuA production at 37°C. The observed thermoregulation is mediated by nucleic acid sequences within the 5′ untranslated region. In addition, we have identified similar nucleotide sequences within the 5′ untranslated region of the orthologous chuA transcript of enteropathogenic E. coli and have demonstrated that it also functions to confer temperature-dependent post-transcriptional regulation. In both function and predicted structure, the regulatory element within the shuA and chuA 5′ untranslated regions closely resembles a FourU RNA thermometer, a zipper-like RNA structure that occludes the Shine-Dalgarno sequence at low temperatures. Increased production of ShuA and ChuA in response to the host body temperature allows for maximal production of these heme acquisition factors within the environment where S. dysenteriae and pathogenic E. coli strains would encounter heme, a host-specific iron source. |
| Databáze: | OpenAIRE |
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