A 3D-psoriatic skin model for dermatological testing: The impact of culture conditions
| Autor: | Alexandra Duque-Fernandez, Lydia Gauthier, Mélissa Simard, Jessica Jean, Isabelle Gendreau, Alexandre Morin, Jacques Soucy, Michèle Auger, Roxane. Pouliot |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Drug medicine.medical_specialty FTIR experiments media_common.quotation_subject Biophysics Pathogenesis Biochemistry Physical characterization of the stratum corneum lcsh:Biochemistry 03 medical and health sciences Psoriatic skin 3D-psoriatic skin model Skin substitutes Stratum corneum medicine Serum deprivation lcsh:QD415-436 Drug toxicity lcsh:QH301-705.5 media_common integumentary system business.industry Percutaneous absorption Dermatology 3. Good health 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Culture conditions business Research Article |
| Zdroj: | Biochemistry and Biophysics Reports, Vol 8, Iss 4, Pp 268-276 (2016) Biochemistry and Biophysics Reports |
| ISSN: | 2405-5808 |
| DOI: | 10.1016/j.bbrep.2016.09.012 |
| Popis: | Background Inadequate representation of the human tissue environment during a preclinical screen can result in inaccurate predictions of compound effects. Consequently, pharmaceutical investigators are searching for preclinical models that closely resemble original tissue for predicting clinical outcomes. Methods The current research aims to compare the impact of using serum-free medium instead of complete culture medium during the last step of psoriatic skin substitute reconstruction. Skin substitutes were produced according to the self-assembly approach. Results Serum-free conditions have no negative impact on the reconstruction of healthy or psoriatic skin substitutes presented in this study regarding their macroscopic or histological appearances. ATR-FTIR results showed no significant differences in the CH2 bands between psoriatic substitutes cultured with or without serum, thus suggesting that serum deprivation did not have a negative impact on the lipid organization of their stratum corneum. Serum deprivation could even lead to a better organization of healthy skin substitute lipids. Percutaneous analyses demonstrated that psoriatic substitutes cultured in serum-free conditions showed a higher permeability to hydrocortisone compared to controls, while no significant differences in benzoic acid and caffeine penetration profiles were observed. Conclusions Results obtained with this 3D-psoriatic skin substitute demonstrate the potential and versatility of the model. It could offer good prediction of drug related toxicities at preclinical stages performed in order to avoid unexpected and costly findings in the clinic. General significance Together, these findings offer a new approach for one of the most important challenges of the 21st century, namely, prediction of drug toxicity. Highlights • Impact of serum-free conditions during psoriatic skin substitutes reconstruction. • Lipids disorganization of healthy and psoriatic skin substitutes. • Permeation profiles of healthy skin substitutes. • Permeation profiles of psoriatic skin substitutes. • Potential and veratility of a 3d-reconstructed model to perform dermatological testing. |
| Databáze: | OpenAIRE |
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