Gel-electromembrane extraction of peptides: Determination of five hypothalamic agents in human plasma samples
| Autor: | Saeed Nojavan, Saied Saeed Hosseiny Davarani, Ahmad Pourahadi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Peptide
02 engineering and technology Octreotide 01 natural sciences Analytical Chemistry Gonadotropin-Releasing Hormone chemistry.chemical_compound medicine Humans Detection limit chemistry.chemical_classification Triptorelin Pamoate Chromatography Chemistry Electromembrane extraction 010401 analytical chemistry Electrochemical Techniques 021001 nanoscience & nanotechnology Healthy Volunteers 0104 chemical sciences Membrane Cetrorelix Human plasma Goserelin Agarose Peptides Somatostatin 0210 nano-technology Gels medicine.drug |
| Zdroj: | Talanta. 217:121025 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2020.121025 |
| Popis: | Agarose gel as a green membrane has been proposed for use in electromembrane extraction of five hypothalamic-related peptides without an ionic carrier. Octreotide, goserelin, triptorelin, cetrorelix, and somatostatin were extracted from 5.0 mL of sample solution (adjusted to pH 5.0) into a microvolume acceptor solution (HCl, 100 mM) under the applied voltage of 30 V in 15 min. The pH of the agarose gel 3.0% (w/v) was adjusted to 4.0 to facilitate the movement of peptides through the membrane. Quantification was performed using an HPLC-UV system on a C18 column. Quantification and detection limits were found to be in the range of 15.0–20.0 ng mL−1 and 4.5–6.0 ng mL−1, respectively. Dynamic linear ranges were found to be in the range of 15.0–1000 ng mL−1 (R2 > 0.995) and recoveries were in the range of 62.3–77.6%. The optimized method was applied to spiked human plasma samples. The method showed relative recoveries in the range of 44.8–66.0%. Finally, the proposed method was compared with and shown to have higher recoveries than, the conventional electromembrane extraction method for the peptides under study. |
| Databáze: | OpenAIRE |
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