Effect of silver doping on antidiabetic and antioxidant potential of ZnO nanorods
| Autor: | Prissana Robkhob, Sougata Ghosh, Dhiraj A. Jamdade, I-Ming Tang, S. Thongmee, Jayesh R. Bellare |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Circular dichroism
Silver Antioxidant Swine Radical medicine.medical_treatment 010501 environmental sciences Nitric Oxide medicine.disease_cause 01 natural sciences Biochemistry Antioxidants Nitric oxide Inorganic Chemistry Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Picrates Superoxides medicine Animals Hypoglycemic Agents Glycoside Hydrolase Inhibitors Amylase Enzyme Inhibitors Pancreas 0105 earth and related environmental sciences chemistry.chemical_classification Reactive oxygen species Nanotubes biology Biphenyl Compounds alpha-Glucosidases Free Radical Scavengers Intestines Enzyme chemistry Amylases biology.protein Molecular Medicine Zinc Oxide 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Journal of Trace Elements in Medicine and Biology. 58:126448 |
| ISSN: | 0946-672X |
| DOI: | 10.1016/j.jtemb.2019.126448 |
| Popis: | Background Increasing resistance to available drugs and their associated side-effects have drawn wide attention towards designing alternative therapeutic strategies for control of hyperglycemia and oxidative stress. The roles of the sizes and shapes of the nanomaterials used in the treatment and management of Type 2 Diabetes Mellitus (T2DM) in preventing chronic hyperglycaemia and oxidative stress are investigated. We report specifically on the effects of doping silver (Ag) into the ZnO nanorods (ZnO:Ag NR’s) as a rational drug designing strategy. Methods Inhibition of porcine pancreatic α-amylase, murine pancreatic amylase, α-glucosidase, murine intestinal glucosidase and amyloglucosidase are checked for evaluation of antidiabetic potential. In addition, the radical scavenging activities of ZnO:Ag NR’s against nitric oxide, DDPH and superoxide radicals are evaluated. Results Quantitative radical scavenging and metabolic enzyme inhibition activities of ZnO:Ag NR’s at a concentration of 100 μg/mL were found to depend on the amount of Ag doped in up to a threshold level (3–4 %). Circular dichroism analysis revealed that the interaction of the NR’s with the enzymes altered their secondary conformation. This alteration is the underlying mechanism for the potent enzyme inhibition. Conclusions Enhanced inhibition of enzymes and scavenging of free radicals primarily responsible for reactive oxygen species (ROS) mediated damage, provide a strong scientific rationale for considering ZnO:Ag NR’s as a candidate nanomedicine for controlling postprandial hyperglycaemia and the associated oxidative stress. |
| Databáze: | OpenAIRE |
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