Mitotic inactivation of a human SWI/SNF chromatin remodeling complex
| Autor: | P. T. Stukenberg, Robert E. Kingston, Saïd Sif, Marc W. Kirschner |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Mitogen-Activated Protein Kinase 3
Macromolecular Substances cells genetic processes Mitosis macromolecular substances Chromatin remodeling Cell Line Genetics Phosphoprotein Phosphatases Humans Chromatin structure remodeling (RSC) complex Phosphorylation biology SWI/SNF complex Base Sequence Cell Cycle DNA Helicases Nuclear Proteins Cell cycle Molecular biology SWI/SNF Chromatin Cell biology Nucleosomes DNA-Binding Proteins enzymes and coenzymes (carbohydrates) Calcium-Calmodulin-Dependent Protein Kinases biology.protein biological phenomena cell phenomena and immunity Mitogen-Activated Protein Kinases Developmental Biology Plasmids Transcription Factors Research Paper |
| Zdroj: | Genesdevelopment. 12(18) |
| ISSN: | 0890-9369 |
| Popis: | During mitosis, chromatin is condensed into mitotic chromosomes and transcription is inhibited, processes that might be opposed by the chromatin remodeling activity of the SWI/SNF complexes. Brg1 and hBrm, which are components of human SWI/SNF (hSWI/SNF) complexes, were recently shown to be phosphorylated during mitosis. This suggested that phosphorylation might be used as a switch to modulate SWI/SNF activity. Using an epitope-tag strategy, we have purified hSWI/SNF complexes at different stages of the cell cycle, and found that hSWI/SNF was inactive in cells blocked in G2–M. Mitotic hSWI/SNF contained Brg1 but not hBrm, and was phosphorylated on at least two subunits, hSWI3 and Brg1. In vitro, active hSWI/SNF from asynchronous cells can be phosphorylated and inactivated by ERK1, and reactivated by dephosphorylation. hSWI/SNF isolated as cells traversed mitosis regained activity when its subunits were dephosphorylated either in vitro or in vivo. We propose that this transitional inactivation and reactivation of hSWI/SNF is required for formation of a repressed chromatin structure during mitosis and reformation of an active chromatin structure as cells leave mitosis. |
| Databáze: | OpenAIRE |
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