Bta-miR-24-3p Controls the Myogenic Differentiation and Proliferation of Fetal, Bovine, Skeletal Muscle-Derived Progenitor Cells by Targeting ACVR1B
| Autor: | Ling Ren, Lingyang Xu, Yahui Wang, Xing Fu, Xin Hu, Lupei Zhang, Junya Li, Luc Willems, Qian Li, Qiyuan Yang, Yishen Xing |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cellular differentiation
proliferation Biology 03 medical and health sciences 0302 clinical medicine RNA interference Gene expression microRNA lcsh:Zoology medicine fetal skeletal muscle lcsh:QL1-991 Progenitor cell ACVR1B 030304 developmental biology 0303 health sciences lcsh:Veterinary medicine General Veterinary Myogenesis bovine Skeletal muscle differentiation Cell biology acvr1b medicine.anatomical_structure bta-mir-24-3p 030220 oncology & carcinogenesis embryonic structures lcsh:SF600-1100 Animal Science and Zoology |
| Zdroj: | Animals, Vol 9, Iss 11, p 859 (2019) Animals Volume 9 Issue 11 |
| ISSN: | 2076-2615 |
| Popis: | MicroRNAs modulate a variety of cellular events, including skeletal muscle development, but the molecular basis of their functions in fetal bovine skeletal muscle development is poorly understood. In this study, we report that bta-miR-24-3p promotes the myogenic differentiation of fetal bovine PDGFR&alpha progenitor cells. The expression of bta-miR-24-3p increased during myogenic differentiation. Overexpression of bta-miR-24-3p significantly promoted myogenic differentiation, but inhibited proliferation. A dual-luciferase assay identified ACVR1B as a direct target of bta-miR-24-3p. Similarly, knocking down ACVR1B by RNA interference also significantly inhibited proliferation and promoted the differentiation of bovine PDGFR&alpha progenitor cells. Thus, our study provides a mechanism in which bta-miR-24-3p regulates myogenesis by inhibiting ACVR1B expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |