Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix
| Autor: | Drago Skrtic, Gili Kaufman |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Confocal Immunocytochemistry Cell Gingiva Biology Extracellular matrix Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Dental Pulp Cell Line Transformed Mesenchymal stem cell Spheroid Cell Biology General Medicine Anatomy Fibroblasts In vitro Extracellular Matrix 030104 developmental biology medicine.anatomical_structure Biophysics Immortalised cell line 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Tissue and Cell. 49:401-409 |
| ISSN: | 0040-8166 |
| Popis: | Cells sensing changes in their microenvironmental stiffness and composition alter their responses, accordingly. This study determines whether gingival fibroblasts (GFs) and dental pulp mesenchymal stem cells (DPMSCs) support the formation of continuous layers in vitro by mimicking the stiffness and protein composition of their native extracellular matrix (ECM). Immortalized cells were incubated with (i) 0-100% Matrigel-ECM (M-ECM) for 7-28d, and with (ii) collagen and fibrin matrices for 14d. Cultures were analyzed by phase-contrast, fluorescence and confocal microscopies. The diameters and surface areas were measured via ImageJ. Self-renewal markers were detected by RT-PCR and immunocytochemistry assays. GFs and DPMSCs developed spheroids interconnected by elongated cell bundles or layers, respectively, expressing the self-renewal markers. Increased matrix stiffness resulted in spheroids replacement by the interconnecting cells/layers. Both cells required 100% M-ECM to reduce their spheroid diameter. However, it reduced the surface area of the interconnecting layers. Those differences led to extended, spindle-shaped GFs vs. compact, ring-shaped DPMSCs constructs. Collagen and fibrin matrices developed continuous layers of tightly connected cells vs. distinctive scattered cell aggregates, respectively. The ability of GFs and DPMSCs to create tissue-like multicellular layers at various matrix conditions may be imprinted by cells' adaptation to mechanical forces and composition in vivo. |
| Databáze: | OpenAIRE |
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