Production of bioactive peptide hydrolysates from deer, sheep, pig and cattle red blood cell fractions using plant and fungal protease preparations
Autor: | Alaa El-Din A. Bekhit, Sonya Mros, Alan Carne, Clara Shui Fern Bah, Michelle McConnell |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Bromelain Proteases Erythrocytes Antioxidant Oxygen radical absorbance capacity Protein Hydrolysates Swine medicine.medical_treatment Biology Antioxidants Hydrolysate Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology medicine Animals Sheep Protease Deer Fungi 04 agricultural and veterinary sciences General Medicine 040401 food science Anti-Bacterial Agents Papain Red blood cell 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry Cattle Peptide Hydrolases Food Science |
Zdroj: | Food Chemistry. 202:458-466 |
ISSN: | 0308-8146 |
Popis: | Protease preparations from plant (papain and bromelain) and fungal (FP400 and FPII) sources were used to hydrolyze the red blood cell fractions (RBCFs) separated from deer, sheep, pig, and cattle abattoir-sourced blood. After 1, 2, 4 and 24 h of hydrolysis, the antioxidant and antibacterial activities of the peptide hydrolysates obtained were investigated. The increase in trichloroacetic acid-soluble peptides over the hydrolysis period was examined using the o-phthaldialdehyde (OPA) assay and the hydrolysis profiles were illustrated using SDS–PAGE. Papain generated RBCF hydrolysates exhibited higher ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) compared to those generated with bromelain, FP400 and FPII. At certain concentrations, 24 h hydrolysates of RBCF using FP400 and FPII were able to inhibit the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results indicated that the use of proteases from plant or fungal sources can produce animal blood hydrolysates with antioxidant and antimicrobial activities. |
Databáze: | OpenAIRE |
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