Nerve conduction velocity as a non-destructive biomarker in the earthworm Aporrectodea caliginosa exposed to insecticides

Autor: Martin Wellby, Ravi Gooneratne, Christophe Mazzia, Yvan Capowiez, Kiran Munir, Magali Rault
Přispěvatelé: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Lincoln University, New Zealand, Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Carbamate
Veterinary medicine
Insecticides
Aché
Health
Toxicology and Mutagenesis
medicine.medical_treatment
Neural Conduction
010501 environmental sciences
Ecotoxicology
01 natural sciences
chemistry.chemical_compound
medicine
Neurotoxicity
Environmental Chemistry
Animals
Soil Pollutants
Earthworms
Oligochaeta
Nerve conduction velocity
0105 earth and related environmental sciences
biology
Dose-Response Relationship
Drug
Environmental Biomarkers
Earthworm
Organophosphate
Agriculture
04 agricultural and veterinary sciences
General Medicine
Pesticide
Aporrectodea caliginosa
biology.organism_classification
Pollution
Acetylcholinesterase
Enzyme assay
language.human_language
Pyrimidines
chemistry
040103 agronomy & agriculture
biology.protein
language
0401 agriculture
forestry
and fisheries
Carbamates
Chlorpyrifos
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Zdroj: Environmental Science and Pollution Research
Environmental Science and Pollution Research, Springer Verlag, 2018, 25 (24), pp.24362-24367. ⟨10.1007/s11356-018-2469-1⟩
Environmental Science and Pollution Research, 2018, 25 (24), pp.24362-24367. ⟨10.1007/s11356-018-2469-1⟩
ISSN: 0944-1344
1614-7499
DOI: 10.1007/s11356-018-2469-1⟩
Popis: International audience; Earthworms are important and useful soil organisms, but in agricultural soils, they are potentially exposed to a wide variety of pesticides. Insecticides represent the highest threat to earthworms and many are neurotoxic. There is a need for a reliable, relevant, simple biomarker to assess the sub-lethal effects of neurotoxic insecticides on earthworms under laboratory or field conditions. The Aporrectodea caliginosa earthworms were exposed to 0 (control), 0.5×, 1× (normal field application rate), and 5× concentrations of a carbamate (Pirimor®) and an organophosphate (Lorsban®) insecticides. The nerve conduction velocity (NCV) of the medial giant fibers of A. caliginosa earthworm was recorded on days 0, 1, 2, 3, 4, and 7 to quantify sub-lethal neurotoxic effects. Acetylcholinesterase (AChE) enzyme activity of A. caliginosa homogenates was measured at the conclusion of the experiment. Pirimor® but not Lorsban® induced a significant decrease in NCV on days 3, 4, and 7 at 1× and 5× doses. A significant dose-dependent decrease was observed on AChE activity to Pirimor® at the doses used but not Lorsban®. A clear relationship is observed between AChE activity and NCV in the case of Pirimor®. This study showed that NCV is a sensitive biomarker that correlates well with classical biomarker measurements such as AChE enzyme activity. This technique could be used to study the impact of insecticides on earthworms and also their recovery.
Databáze: OpenAIRE
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