Experimental glomerulonephritis induced by in situ formation of immune complexes in glomerular capillary wall
| Autor: | Steven M. Golbus, Curtis B. Wilson |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Male
In situ medicine.medical_specialty Kidney Glomerulus Fluorescent Antibody Technique Antigen-Antibody Complex Experimental glomerulonephritis Glomerulonephritis Immune system Internal medicine Concanavalin A medicine Animals Glycoproteins Kidney biology Chemistry Lectin medicine.disease Molecular biology Capillaries Rats Glomerular capillary Disease Models Animal medicine.anatomical_structure Endocrinology Nephrology Antibody Formation biology.protein Binding Sites Antibody |
| Zdroj: | Kidney International. 16:148-157 |
| ISSN: | 0085-2538 |
| DOI: | 10.1038/ki.1979.116 |
| Popis: | Experimental glomerulonephritis induced by in situ formation of immune complexes in glomerular capillary wall. An experimental model of glomerulonephritis was produced by the in situ formation of immune complexes directly in the glomerular capillary wall. Perfusing the lectin concanavalin A (Con A) into the left renal arteries of rats led to its binding diffusely to the glycoproteins of the glomerular capillary wall of only that kidney in each animal. The subsequent reaction with anti-Con A antibody (either administered systemically or actively induced) resulted in an exudative and proliferative glomerulonephritis confined to the Con A perfused kidney. Immunofluorescence disclosed the diffuse deposition of immunoglobulin, Con A, and C3 in the perfused, but not the unperfused kidney. The quantitative relationship between antigen and antibody binding and histologic outcome was determined. Since lectins have been found in mammalian tissues, as well as in infectious agents that are pathogenic in man, a series of events conceptually similar to this in situ model may occur in some cases of glomerulonephritis in man. Glomerulonephrite experimentale determinee par la formation in situ de complexes immuns dans la paroi du capillaire glomerulaire. Un modele experimental de glomerulonephrite a ete produit par la formation in situ de complexes immuns dans la paroi du capillaire glomerulaire. La perfusion de concavaline A (Con A) dans les arteres renales gauches de rats entraine la liaison diffuse du produit aux glycoproteines de la paroi du capillaire glomerulaire seulement dans le rein injecte. Les reactions ulterieures avec un anticorps anti-Con A (soit administre par voie systemique, soit induit activement) ont pour consequence une glomerulonephrite exsudative et proliferative localisee au seul rein injecte. L'immunofluorescence montre des depots diffus d'immunoglobuline, de Con A, et de C3 dans le rein perfuse mais non dans le rein oppose. Les relations quantitatives entre la liaison et l'evolution histologique ont ete determinees. Du fait que des lectines ont ete trouvees aussi bien dans les tissus des mammiferes que dans des agents infectieux pathogenes pour l'homme, il est possible qu'une sequence d'evenements conceptuellement similaire a ce modele in situ puisse survenir dans certains cas de glomerulonephrite chez l'homme. |
| Databáze: | OpenAIRE |
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