Low-dose ketamine pretreatment reduces oxidative damage and inflammatory response following CO2 pneumoperitoneum in rats
| Autor: | Li Ning, Zheng Peng, Ding Bowen, Li Jieshou, Xu Xingwei, Ji Wu, Feng Tao, Gao Xin, Kao Xiaoming |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment medicine.disease_cause Sevoflurane Rats Sprague-Dawley chemistry.chemical_compound Pneumoperitoneum Malondialdehyde Internal medicine medicine Animals Ketamine Saline Interleukin-6 Tumor Necrosis Factor-alpha business.industry General Medicine Carbon Dioxide medicine.disease Rats Oxidative Stress Endocrinology chemistry Reperfusion Injury Anesthesia Anesthetic business Reperfusion injury Oxidative stress medicine.drug |
| Zdroj: | Clinical & Investigative Medicine. 37:124 |
| ISSN: | 1488-2353 |
| DOI: | 10.25011/cim.v37i3.21379 |
| Popis: | Purpose: The duration of pneumoperitoneum during laparoscopic procedures may contribute to post-surgical oxidative stress. Previous studies have shown that low-dose ketamine, an anesthetic with anti-inflammatory properties, protects various organs from ischemia-reperfusion injury. This study investigated the effects of low-dose ketamine on the overproduction of oxidants and the tissue damage caused by intra-abdominal pressure during CO2 pneumoperitoneum. Methods: Male Sprague Dawley rats received a CO2 pneumoperitoneum of 15 mmHg and preceded by either low-dose ketamine (KP1, 5 mg/kg; KP2, 10 mg/kg) or 0.9% saline (PR, 3 ml). General anethesia was provided by pentobarbital and sevoflurane. The control group (CR) received an intraperitoneal saline injection and sham surgery. Three hours after pneumoperitoneum, serum concentrations of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), superoxide dismutase (SOD) and intestinal fatty acid binding protein (iFABP) were measured and liver, kidney, lung, and intestine were evaluated for tissue damage. Results: The highest plasma MDA, TNF-α, IL-6 and iFABP values were observed at T1 (after 3 hours of pneumoperitoneum) in the PR group, followed by the KP1, KP2, and CR groups (P < 0.01). SOD concentrations showed an opposite trend and were highest in the CR group, followed by the KP2, KP1, and PR groups (P < 0.01). TNF-α concentration was significantly lower in the KP2 than the KP1 group (P < 0.05). Histopathologic scoring of organ sections demonstrated the lowest scores in the KP2 group, followed by the KP1 and PR groups, in an increasing order (P < 0.05). Conclusion: Pretreatment with low-dose ketamine before general anaesthesia protects against potential oxidative damage and inflammatory response caused by CO2 pneumoperitoneum. |
| Databáze: | OpenAIRE |
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